Previous studies on the vitrification of peach palm (Bactris gasipaes Kunth) embryogenic cluster highlighted PVS3 as essential for survival, but toxic nonetheless. The objective of this work was to identify methods to improve embryogenic cluster survival through either modification to PVS3 or other changes to the established protocol. PVS3 reduced to 80% strength was less toxic to non-vitrified controls and showed no significant difference in vitrified embryogenic cluster at any incubation time (60, 120, 180, or 240 min) compared to full strength PVS3. The addition of 0.1 M KCl, MgCl₂, MgSO₄, or K₂H(PO₄) to 80% PVS3 increased vitrified embryogenic cluster regrowth. D₂O had no significant effect on embryogenic cluster regrowth when used as a solvent for PVS3 compared to H₂O. Larger embryogenic cluster showed greater regrowth than smaller ones. Regrowth increased with rising rewarming temperature up to 70 °C, the highest tested temperature. The duration of rewarming between 1, 2, or 3 min had no overall significant effect, suggesting that the most critical events in rewarming occur within the first minute. The most effective strips used for droplet vitrification were made from aluminum or silver, which were significantly more effective than polystyrene and expanded polystyrene. Droplet vitrification was initially more effective than conventional cryovial immersion vitrification at 60 min incubation in PVS3, but not significantly different at 120, 180 or 240 min incubations. Partial dehydration for 1 h in a laminar flow hood significantly increased regrowth, but longer dehydration times decreased regrowth when combines with longer PVS3 incubation times.

先前对桃棕榈（Bactris gasipaes Kunth）胚发生簇的玻璃化的研究强调PVS3对存活至关重要，但仍具有毒性。这项工作的目的是确定通过修改PVS3或对既定方案进行其他更改来提高胚胎发生簇存活的方法。与全强度PVS3相比，PVS3降低至80％的强度对非玻璃化对照的毒性较小，并且在任何孵育时间（60、120、180或240分钟）的玻璃化胚发生簇中均无显着差异。向80％PVS3中添加0.1 M KCl，MgCl ₂，MgSO ₄或K ₂ H（PO ₄）可增加玻璃化的胚胎发生簇的再生长。第_2天与H ₂相比，O用作PVS3的溶剂时，对胚发生簇再生长没有显着影响O.较大的胚发生簇显示出比较小的更大的再生长。随着温度升高到70°C（最高测试温度），再生长增加。1分钟，2分钟或3分钟之间的变暖持续时间没有整体上的显着影响，表明变暖中最关键的事件发生在第一分钟之内。用于液滴玻璃化的最有效条带由铝或银制成，它们比聚苯乙烯和发泡聚苯乙烯有效得多。最初，在PVS3中孵育60分钟后，液滴玻璃化比常规的冷冻小管浸入玻璃化更有效，但在120、180或240分钟的孵育中，液滴玻璃化没有显着差异。在层流通风橱中部分脱水1小时可显着增加再生长，