Keeping in view the increasing importance of botanical insecticides, the various metabolites from different plants are being investigated for insecticidal activity. Alpinia galanga , a medicinal plant belonging to family Zingiberaceae exhibits a wide range of biological activities. In the present study, purified compounds of A. galanga i.e. 1′-acetoxychavicol acetate and galangin were evaluated for their effect on detoxifying enzymes viz. esterases, acid phosphatases, alkaline phosphatses and glutathione-S-transferases as well as DNA damage of the Spodoptera litura (Fab.) larvae. DNA damaging potential of both the compounds was ascertained using comet assay. The purified compounds significantly changed the enzymatic profile of S. litura as well as induced genotoxic effects. Change in enzyme activity and damage to DNA may lead to genetic and physiological changes in insect and ultimately affect its fitness and survival.

中文翻译：

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高良姜纯化化合物对多食性鳞翅目害虫斜纹夜蛾 (Fabricius) 的遗传毒性和生化作用的评估

考虑到植物性杀虫剂的重要性日益增加，正在研究来自不同植物的各种代谢物的杀虫活性。高良姜属姜科药用植物，具有广泛的生物活性。在本研究中，评估了高良姜的纯化化合物，即 1'-乙酰氧基茶酚乙酸酯和高良姜素对解毒酶的影响。酯酶、酸性磷酸酶、碱性磷酸酶和谷胱甘肽-S-转移酶以及斜纹夜蛾 (Fab.) 幼虫的 DNA 损伤。使用彗星试验确定了这两种化合物的 DNA 损伤潜力。纯化的化合物显着改变了斜纹夜蛾的酶谱以及诱导的基因毒性作用。