Brucella ovis infection results in genital damage and epididymitis in rams, placental inflammation and rare abortion in ewes, and neonatal mortality in lambs. However, the mechanism underlying B. ovis infection remains unclear. In the present study, we used prokaryotic transcriptome sequencing to identify the differentially expressed genes (DEGs) between wild-type B. ovis and intracellular B. ovis in RAW264.7 macrophages. Gene ontology (GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed, and quantitative reverse transcriptase PCR (qRT-PCR) was used to validate the top 10 upregulated and downregulated DEGs. The results showed that 212 genes were differentially expressed, including 68 upregulated and 144 downregulated genes, which were mainly enriched in 30 GO terms linked to biological process, cellular component, and molecular function. KEGG analysis showed that the DEGs were enriched in the hypoxia-inducible factor 1 (HIF-1) signaling pathway, mitogen-activated protein kinase (MAPK) signaling pathway, beta-alanine metabolism, and quorum sensing pathway. BME_RS01160, BME_RS04270, BME_RS08185, BME_RS12880, BME_RS25875, predicted_RNA865, and predicted_RNA953 were confirmed with the transcriptome sequencing data. Hence, our findings not only reveal the intracellular parasitism of B. ovis in the macrophage immune system, but also help to understand the mechanism of chronic B. ovis infection.

中文翻译：

---

在RAW264.7巨噬细胞免疫系统中存活的细胞内布鲁氏菌的转录组景观。

绵羊布鲁氏菌感染会导致公羊的生殖器损伤和附睾炎，母羊的胎盘发炎和罕见的流产，以及羔羊的新生儿死亡率。但是，尚不了解卵圆芽孢杆菌感染的潜在机制。在本研究中，我们使用原核转录组测序来鉴定RAW264.7巨噬细胞中野生型B. ovis和细胞内B. ovis之间的差异表达基因（DEG）。进行了基因本体论（GO）术语富集和《京都议定书》的基因和基因组百科全书（KEGG）途径分析，并使用定量逆转录酶PCR（qRT-PCR）来验证前10个上调和下调的DEG。结果表明，差异表达212个基因，其中68个上调基因和144个下调基因，主要富集与生物学过程相关的30个GO术语。细胞成分和分子功能。KEGG分析表明，DEGs富含缺氧诱导因子1（HIF-1）信号传导途径，有丝分裂原激活的蛋白激酶（MAPK）信号传导途径，β-丙氨酸代谢和群体感应途径。用转录组测序数据确认了BME_RS01160，BME_RS04270，BME_RS08185，BME_RS12880，BME_RS25875，预测的_RNA865和预测的_RNA953。因此，我们的发现不仅揭示了巨噬细胞免疫系统中牛卵杆菌的胞内寄生性，而且有助于了解慢性牛卵杆菌的感染机制。和仲裁感测路径。用转录组测序数据证实了BME_RS01160，BME_RS04270，BME_RS08185，BME_RS12880，BME_RS25875，预测的_RNA865和预测的_RNA953。因此，我们的发现不仅揭示了巨噬细胞免疫系统中牛卵杆菌的胞内寄生性，而且有助于了解慢性牛卵杆菌的感染机制。和仲裁感测路径。用转录组测序数据证实了BME_RS01160，BME_RS04270，BME_RS08185，BME_RS12880，BME_RS25875，预测的_RNA865和预测的_RNA953。因此，我们的发现不仅揭示了巨噬细胞免疫系统中牛卵杆菌的胞内寄生性，而且有助于了解慢性牛卵杆菌的感染机制。