The RNA extraction was performed from foliar (F) and whole wheat plants (including rhizosphere) (WP) samples by (1) the standard TRIzol® protocol, and (2) a modified CTAB and TRIzol® protocol. The modified CTAB and TRIzol® protocol was able to extract high-quality RNA (205.96 ± 18.7 ng/µL for F, and 231.76 ± 66.8 ng/µL for WP; RIN > 8.0), compared to the standard TRIzol® protocol (92.73 ± 24.2 ng/µL for F, and WP completely degraded, RIN < 8.0). Real-time RT-PCR assay was carried out for 6-SFT1 (target) and 18S rRNA (housekeeping) genes, which showed a PCR efficiency of 111% and 118%, respectively, and a no significant relative expression (3773 ± 1383.8 for F and 2847 ± 1037.5 for WP) was observed from RNA extracted by the modified protocol. The modified CTAB and TRIzol® protocol was able to produce high-quality RNA (yield, purity, and integrity) from foliar and whole wheat plants (including rhizosphere with recalcitrant properties).

中文翻译：

---

用于从全麦幼苗（包括根际）中提取高质量 RNA 的改良 CTAB 和 Trizol® 方案

使用 (1) 标准 TRIzol® 方案和 (2) 修改后的 CTAB 和 TRIzol® 方案从叶 (F) 和全麦植物（包括根际）(WP) 样品中提取 RNA。与标准 TRIzol® 方案 (92.73 ± 8.0) 相比，改进的 CTAB 和 TRIzol® 方案能够提取高质量的 RNA（F 为 205.96 ± 18.7 ng/µL，WP 为 231.76 ± 66.8 ng/µL；RIN > 8.0） F 为 24.2 ng/µL，WP 完全降解，RIN < 8.0）。对 6-SFT1（靶点）和 18S rRNA（管家）基因进行实时 RT-PCR 检测，PCR 效率分别为 111% 和 118%，无显着相对表达（3773±1383.8 F 和 WP 的 2847 ± 1037.5) 从修改后的协议提取的 RNA 中观察到。修改后的 CTAB 和 TRIzol® 方案能够产生高质量的 RNA（产量、纯度、