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Expression characteristics of immune factors in secondary lymphoid organs of newborn, juvenile and adult yaks (Bos grunniens)
Cell and Tissue Research ( IF 3.6 ) Pub Date : 2020-05-19 , DOI: 10.1007/s00441-020-03219-4
Yufeng Huang 1 , Yan Cui 1, 2 , Sijiu Yu 2 , Penggang Liu 1 , Jun Liu 1 , Jun-Feng He 1 , Juan Sun 1
Affiliation  

Little is known about lymphoid organ development in yaks. In this study, we characterize and evaluate the main markers of T cell, B cell, plasma cell and antigen-presenting cell in the mesenteric lymph nodes, spleen and hemal node in newborn, juvenile and adult yaks by immunohistochemistry, real-time quantitative polymerase chain reaction and western blotting. The structures of all organs were not fully developed in newborn. The CD3+ cells were mainly located in the paracortex area of the mesenteric lymph node and the T cell dependent area in the hemal node and spleen. CD79a+ cells were mainly detected in the lymphoid follicles. The expression of CD3 and CD79a increased from newborn to juvenile and then decreased in adults. The expression of CD3 was always higher in the spleen and CD79a was higher in the mesenteric lymph node. IgG+ and IgA+ cells were observed in all examined samples, except in newborn yak hemal node. IgG and IgA were up-regulated with age and the highest expression was observed in the mesenteric lymph node. The SIRPα and CD68 were widely expressed. A significant feature was that the SIRPα expression in the spleen was lowest in newborns but highest in juvenile and adult yaks. The expression of CD68 in the hemal node was highest in all groups and increased from newborn to adult yaks. This study sheds light on the relationship between the morphology and function of these organs and provides useful references for normal yak lymphoid organ development.

中文翻译:

新生、幼年和成年牦牛(Bos grunniens)次级淋巴器官中免疫因子的表达特征

关于牦牛的淋巴器官发育知之甚少。在本研究中,我们通过免疫组织化学、实时定量聚合酶表征和评估新生儿、幼年和成年牦牛肠系膜淋巴结、脾脏和血管淋巴结中 T 细胞、B 细胞、浆细胞和抗原呈递细胞的主要标志物。链反应和蛋白质印迹。新生儿所有器官的结构均未发育完全。CD3+细胞主要位于肠系膜淋巴结的副皮质区和血管淋巴结和脾脏的T细胞依赖区。CD79a+ 细胞主要在淋巴滤泡中检测到。CD3 和 CD79a 的表达从新生儿到幼年增加,然后在成人中减少。CD3在脾脏中的表达始终较高,CD79a在肠系膜淋巴结中的表达较高。在所有检查的样本中都观察到了 IgG+ 和 IgA+ 细胞,但新生牦牛血管结除外。IgG 和 IgA 随着年龄的增长而上调,并且在肠系膜淋巴结中观察到最高表达。SIRPα和CD68广泛表达。一个显着的特征是脾脏中的 SIRPα 表达在新生儿中最低,但在幼年和成年牦牛中最高。CD68在血管结节中的表达在所有组中最高,并且从新生儿到成年牦牛增加。该研究阐明了这些器官的形态和功能之间的关系,并为正常牦牛淋巴器官的发育提供了有益的参考。SIRPα和CD68广泛表达。一个显着的特征是脾脏中的 SIRPα 表达在新生儿中最低,但在幼年和成年牦牛中最高。CD68在血管结节中的表达在所有组中最高,并且从新生儿到成年牦牛增加。该研究阐明了这些器官的形态和功能之间的关系,并为正常牦牛淋巴器官的发育提供了有益的参考。SIRPα和CD68广泛表达。一个显着的特征是脾脏中的 SIRPα 表达在新生儿中最低,但在幼年和成年牦牛中最高。CD68在血管结节中的表达在所有组中最高,并且从新生儿到成年牦牛增加。该研究阐明了这些器官的形态和功能之间的关系,并为正常牦牛淋巴器官的发育提供了有益的参考。
更新日期:2020-05-19
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