The present study was aimed to consider the effect of light intensity on the growth and lipid accumulation properties of a freshwater microalga Chlorococcum oleofaciens KF584224.1. The microalga was cultivated at five different light intensities of 50, 100, 200, 400, and 800 μmol photons m⁻² s⁻¹ for 20 days. The culture curves illustrated the fastest growth rate for microalgae illuminated with 200 μmol photons m⁻² s⁻¹, while the longest exponential growth curve was obtained in culture illuminated with 400 μmol photons m⁻² s⁻¹. Once algae reached the stationary phase, the maximum biomass productivity (367.82 ± 21.63 mg L⁻¹ day⁻¹) was found in culture illuminated with 200 μmol photons m⁻² s⁻¹, while the highest lipid content (59.18 ± 1.62%) and lipid productivity (126.72 ± 3.27 mg L⁻¹ day⁻¹) were achieved in culture illuminated with 400 μmol photons m⁻² s⁻¹. The ratio of saturated fatty acids significantly increased with enhancing light illumination, while the ratio of monounsaturated fatty acids and polyunsaturated fatty acids tend to decline (p < 0.05). The properties of biodiesel obtained from C. oleofaciens cultured under 50 μmol photons m⁻² s⁻¹ met the specifications provided by the international biodiesel standards (European EN 14214 and US ASTM D6751), higher light illumination improved the biodiesel quality. Findings of the present study demonstrated that light intensity could improve the lipid productivity and biodiesel properties obtained from C. oleofaciens as a potential feedstock for biofuel production, especially under 400 μmol photons m⁻² s⁻¹ light intensity.

本研究的目的是考虑光强度对淡水微藻绿藻Chofococcum oleofaciens KF584224.1的生长和脂质积累特性的影响。在50、100、200、400和800μmol光子m ^-2  s ^-1的五种不同光强度下培养微藻20天。培养曲线显示了用200μmol光子m ^-2  s ^-1照射的微藻的最快生长速率，而在用400μmol光子m ^-2  s ^-1照射的培养物中获得了最长的指数生长曲线。藻类进入固定相后，最大生物量生产力（367.82±21.63 mg L ^-1 天在以200μmol光子m ^-2  s ^-1照射的培养物中发现^-1），而在照射的培养物中获得最高的脂质含量（59.18±1.62％）和脂质生产率（126.72±3.27 mg L ^-1  day ^-1）具有400μmol光子m ^-2  s ^-1。饱和脂肪酸的比例随着光照的增加而显着增加，而单不饱和脂肪酸和多不饱和脂肪酸的比例趋于下降（p  <0.05）。得自C的生物柴油的性质。在50μmol光子m ^-2  s ^-1下培养的油橄榄符合国际生物柴油标准（欧洲EN 14214和美国ASTM D6751）提供的规范，更高的光照度改善了生物柴油的质量。本研究的发现表明，光强度可以提高从C获得的脂质生产力和生物柴油特性。油橄榄作为生物燃料生产的潜在原料，尤其是在400μmol光子m ^-2  s ^-1光强度下。