Abstract

In this study, fungal endophytes were screened from samples of Polygonum cuspidatum and 24 endophytes were obtained. The fungal endophytes were allocated to 6 orders and 9 genera by phylogenetic analysis. Based on the ability of fungal endophytes to induce biotransformation, Penicillium sp. F5 was selected for the biotransformation of resveratrol. Fermentation of media using Penicillium sp. F5 yielded transformation products, giving a main product that was purified using silica gel column chromatography. The structure of main product was established as pterostilbene (trans-3,5-dimethoxy-4'-hydroxystilbene) by using ¹H-NMR, ¹³C -NMR, HSQC and HMBC spectroscopy. Pterostilbene plays an important role in resisting disease in humans and shows more metabolic stability, stronger pharmacological activities, and longer half-life when compared with resveratrol.

中文翻译：

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从虎杖中发现内生真菌，并通过内生青霉菌将白藜芦醇生物转化为紫草烯。F5

摘要

在这项研究中，从虎杖样品中筛选出真菌内生菌，并获得了24种内生菌。通过系统进化分析，将真菌内生菌分为6目9属。基于真菌内生菌诱导生物转化的能力，青霉菌。选择F5用于白藜芦醇的生物转化。使用Penicillium sp。发酵培养基。F5产生转化产物，得到主要产物，其使用硅胶柱色谱法纯化。通过¹ H-NMR，¹³建立了主要产物的结构为蝶茂（trans-3,5-dimethoxy-4'-hydroxystilbene）13 C-NMR，HSQC和HMBC光谱。与白藜芦醇相比，紫檀二烯在抵抗人类疾病方面起着重要作用，并显示出更高的代谢稳定性，更强的药理活性和更长的半衰期。