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Tools for successful proliferation: diverse strategies of nutrient acquisition by a benthic cyanobacterium.
The ISME Journal ( IF 11.0 ) Pub Date : 2020-05-18 , DOI: 10.1038/s41396-020-0676-5 H S Tee 1 , D Waite 1 , L Payne 1 , M Middleditch 1 , S Wood 2 , K M Handley 1
The ISME Journal ( IF 11.0 ) Pub Date : 2020-05-18 , DOI: 10.1038/s41396-020-0676-5 H S Tee 1 , D Waite 1 , L Payne 1 , M Middleditch 1 , S Wood 2 , K M Handley 1
Affiliation
Freshwater cyanobacterial blooms have increased worldwide, channeling organic carbon into these systems, and threatening animal health through the production of cyanotoxins. Both toxic and nontoxic Microcoleus proliferations usually occur when there are moderate concentrations of dissolved inorganic nitrogen, but when phosphorus is scarce. In order to understand how Microcoleus establishes thick biofilms (or mats) on riverbeds under phosphorus-limiting conditions, we collected Microcoleus-dominated biofilms over a 19-day proliferation event for proteogenomics. A single pair of nitrogen-dependent Microcoleus species were consistently present in relatively high abundance, although each followed a unique metabolic trajectory. Neither possessed anatoxin gene clusters, and only very low concentrations of anatoxins (~2 µg kg-1) were detected, likely originating from rarer Microcoleus species also present. Proteome allocations were dominated by photosynthesizing cyanobacteria and diatoms, and data indicate biomass was actively recycled by Bacteroidetes and Myxococcales. Microcoleus likely acquired nutrients throughout the proliferation event by uptake of nitrate, urea, and inorganic and organic phosphorus. Both species also harbored genes that could be used for inorganic phosphate solubilization with pyrroloquinoline quinone cofactors produced by cohabiting Proteobacteria. Results indicate that Microcoleus are equipped with diverse mechanisms for nitrogen and phosphorus acquisition, enabling them to proliferate and out-compete others in low-phosphorus waters.
中文翻译:
成功增殖的工具:底栖蓝细菌获取营养的多种策略。
淡水蓝藻在全球范围内大量繁殖,将有机碳引入这些系统,并通过产生蓝藻毒素威胁动物健康。当溶解的无机氮浓度适中但磷缺乏时,有毒和无毒的微鞘藻增殖通常都会发生。为了了解微鞘藻如何在磷限制条件下在河床上建立厚厚的生物膜(或垫),我们收集了为期 19 天的增殖事件中以微鞘藻为主的生物膜用于蛋白质组学。一对依赖氮的微鞘藻物种始终以相对较高的丰度存在,尽管每个物种都遵循独特的代谢轨迹。两者都不具有虾毒素基因簇,并且仅检测到非常低浓度的虾毒素(~2 µg kg-1),可能源自也存在的更稀有的微鞘藻属物种。蛋白质组分配以光合作用蓝细菌和硅藻为主,数据表明拟杆菌门和粘球菌门积极回收生物量。微鞘藻可能在整个增殖过程中通过摄取硝酸盐、尿素以及无机和有机磷来获取营养。这两个物种还都含有可用于与共生变形菌产生的吡咯喹啉醌辅助因子溶解无机磷酸盐的基因。结果表明,微鞘藻具有多种获取氮和磷的机制,使它们能够在低磷水域中增殖并超越其他藻类。
更新日期:2020-05-18
中文翻译:
成功增殖的工具:底栖蓝细菌获取营养的多种策略。
淡水蓝藻在全球范围内大量繁殖,将有机碳引入这些系统,并通过产生蓝藻毒素威胁动物健康。当溶解的无机氮浓度适中但磷缺乏时,有毒和无毒的微鞘藻增殖通常都会发生。为了了解微鞘藻如何在磷限制条件下在河床上建立厚厚的生物膜(或垫),我们收集了为期 19 天的增殖事件中以微鞘藻为主的生物膜用于蛋白质组学。一对依赖氮的微鞘藻物种始终以相对较高的丰度存在,尽管每个物种都遵循独特的代谢轨迹。两者都不具有虾毒素基因簇,并且仅检测到非常低浓度的虾毒素(~2 µg kg-1),可能源自也存在的更稀有的微鞘藻属物种。蛋白质组分配以光合作用蓝细菌和硅藻为主,数据表明拟杆菌门和粘球菌门积极回收生物量。微鞘藻可能在整个增殖过程中通过摄取硝酸盐、尿素以及无机和有机磷来获取营养。这两个物种还都含有可用于与共生变形菌产生的吡咯喹啉醌辅助因子溶解无机磷酸盐的基因。结果表明,微鞘藻具有多种获取氮和磷的机制,使它们能够在低磷水域中增殖并超越其他藻类。