Abstract

The complete mitochondrial genome of Hemerobius spodipennis Yang, 1987 was sequenced in this study. The complete mitochondrial genome is a typical double-stranded circular molecule of 16,343 bp (GenBank accession number: MT268963) comprising of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a control region. The gene order is identical to that of the putative ancestral arrangement of insects and other lacewings. All protein-coding genes initiate with ATN, except COI use CGA as start codons and terminate with TAG or TAA, expect ND5 and ND4 use TA– or a single T–– residue as the stop codon. All tRNAs, ranging from 63 to 72 bp, can be folded into typical clover-leaf secondary structure except for tRNA^Ser(AGN), in which the dihydrouridine (DHU) arm did not form a stable stem-loop structure. The control region is 1433 bp long with an A + T content of 91.4%. In the sampled families of Neuroptera, each family showed a monophyletic cluster and Polystoechotidae + Rapismatidae, Hemerobiidae + (Chrysopidae + (Polystoechotidae + Rapismatidae)), are recovered in phylogenetic analyses with high supports.

摘要

在这项研究中对Hemerobius spodipennis Yang，1987的完整线粒体基因组进行了测序。完整的线粒体基因组是一个典型的16343 bp的双链环状分子（GenBank登录号：MT268963），包含13个蛋白质编码基因，22个转移RNA基因，2个核糖体RNA基因和一个控制区。基因顺序与假定的昆虫和其他草的祖先顺序相同。除COI使用CGA作为起始密码子并以TAG或TAA终止外，所有蛋白质编码基因均以ATN开头，预计为ND5和ND4使用TA–或单个T––残基作为终止密码子。除tRNA ^Ser（AGN）以外，所有tRNA的长度范围为63至72 bp，均可折叠成典型的三叶草叶二级结构，其中二氢尿苷（DHU）臂未形成稳定的茎环结构。对照区长1433 bp，A + T含量为91.4％。在取样的Neuroptera家族中，每个家族均表现出单系聚类，并且在高系统支持的系统发育分析中回收了Polystoechotidae + Rapismatidae，Hemerobiidae +（Chrysopidae +（Polystoechotidae + Rapismatidae））。