ABSTRACT Effects of dietary supplementation of Bifidobacterium longhum, Bifidobacterium thermophilum, Bacillus subtilis, and Lactobacillus acidophilus on growth performance, health condition, body chemical composition, and water quality of Nile tilapia (Oreochromis niloticus) fingerlings were evaluated. Five experimental treatments in triplicate were fed a diet containing 30% crude protein supplemented with variable inclusion of bacterial mixture: control, T1, T2, T3, and T4 containing 0, 1.0, 2.0, 3.0, and 4.0 g bacterial mixture/kg diet, respectively. Fish fed the T4 diet had the highest final body weight, weight gain, specific growth rate, thermal growth coefficient, feed intake, and the best feed conversion ratio. No significant differences in body chemical composition of fish were observed in terms of dry matter, crude protein, lipid, ash, and energy content among all treatments. Probiotic supplementation showed no noticeable differences on haematological and biochemical blood parameters, except for packed cell volume, albumin, albumin/globulin ratio, aspartate transaminase, glucose, uric acid, triglycerides, and cholesterol. Conclusively, Nile tilapia fingerlings diet supplemented with evaluated probiotic with an optimal dietary supplementation of 4 g/kg diet improved growth performance and fish health without a negative impact on water quality. This supplementation could therefore be used as a probiotic in Nile tilapia fingerlings diet.

中文翻译：

---

有益细菌的膳食混合物对尼罗罗非鱼、尼罗罗非鱼鱼种生长性能、健康状况、化学成分和水质的影响

摘要 评估了日粮中添加长双歧杆菌、嗜热双歧杆菌、枯草芽孢杆菌和嗜酸乳杆菌对尼罗罗非鱼（Oreochromis niloticus）鱼种的生长性能、健康状况、身体化学成分和水质的影响。给五个实验处理一式三份喂食含有 30% 粗蛋白的饮食，并辅以可变的细菌混合物：对照、T1、T2、T3 和 T4，含有 0、1.0、2.0、3.0 和 4.0 g 细菌混合物/kg 饮食，分别。饲喂 T4 日粮的鱼具有最高的最终体重、增重、比生长率、热生长系数、采食量和最佳饲料转化率。在干物质、粗蛋白、脂质、灰分、和所有处理中的能量含量。除了细胞体积、白蛋白、白蛋白/球蛋白比率、天冬氨酸转氨酶、葡萄糖、尿酸、甘油三酯和胆固醇外，益生菌补充剂在血液学和生化血液参数方面没有显着差异。最后，尼罗罗非鱼鱼种日粮中添加了经评估的益生菌，最佳日粮添加量为 4 g/kg 日粮，可改善生长性能和鱼类健康，而不会对水质产生负面影响。因此，这种补充剂可用作尼罗罗非鱼鱼种饲料中的益生菌。尿酸、甘油三酯和胆固醇。最后，尼罗罗非鱼鱼种日粮中添加了经评估的益生菌，最佳日粮添加量为 4 g/kg 日粮，可改善生长性能和鱼类健康，而不会对水质产生负面影响。因此，这种补充剂可用作尼罗罗非鱼鱼种饲料中的益生菌。尿酸、甘油三酯和胆固醇。最后，尼罗罗非鱼鱼种日粮中添加了经评估的益生菌，最佳日粮添加量为 4 g/kg 日粮，可改善生长性能和鱼类健康，而不会对水质产生负面影响。因此，这种补充剂可用作尼罗罗非鱼鱼种饲料中的益生菌。