The objective of this study was to determine the minimum inhibitory concentrations (MICs) and pharmacodynamic profiles of four ionophores (lasalocid, monensin, narasin and salinomycin) against staphylococcal isolates from clinical cases of human and veterinary staphylococcal infections, and to determine the effect of methicillin resistance on the antimicrobial activity of ionophores. Broth microdilution MIC testing was used to determine antimicrobial activity against 156 staphylococcal isolates of human and veterinary origin. Pharmacodynamic profiles were examined using time‐kill kinetics profiles against an ATCC type strain of Staphylococcus aureus and a clinical isolate of methicillin‐resistant Staphylococcus pseudintermedius. All tests were performed in accordance with CLSI guidelines. All four ionophores demonstrated antimicrobial activity against methicillin‐resistant staphylococci at concentrations similar to those observed for methicillin‐susceptible isolates of the same species. Testing of human and veterinary MRSA isolates also showed that MIC values were not influenced by the host origin of the isolates. Pharmacodynamic profiles were similar for both isolates tested across all four ionophores, with similar reductions in viable cell counts being observed over an 18‐ to 24‐hr period. Lasalocid, monensin, narasin and salinomycin all demonstrated antimicrobial activity against staphylococcal isolates of human and veterinary origins, with activity being unaffected by methicillin resistance status, although some Staphylococcus species‐specific effects were observed that require further investigation.

中文翻译：

---

四种聚醚离子载体对耐甲氧西林的葡萄球菌属的体外功效和药效学特征

这项研究的目的是确定四种离子载体（拉索洛菌素，莫能菌素，萘灵和沙利霉素）对人和兽类葡萄球菌感染临床病例中分离出的葡萄球菌的最低抑菌浓度（MIC）和药效学特征，并确定甲氧西林的效果对离子载体的抗菌活性有抗药性。肉汤微稀释MIC测试用于确定对人和兽医来源的156种葡萄球菌分离株的抗菌活性。使用针对ATCC型金黄色葡萄球菌和耐甲氧西林的假性金黄色葡萄球菌临床分离株的时间杀灭动力学曲线检查了药效学曲线。所有测试均根据CLSI指南进行。所有四个离子载体在与对相同物种的耐甲氧西林敏感菌株中观察到的浓度相似的情况下，均显示出对耐甲氧西林耐药葡萄球菌的抗菌活性。对人和兽类MRSA分离株的测试还显示，MIC值不受分离株宿主来源的影响。在所有四个离子载体上测试的两种分离物的药效学特征均相似，在18至24小时内观察到存活细胞数的相似减少。Lasalocid，monensin，narasin和salinomycin均对人和兽医来源的葡萄球菌分离物均具有抗菌活性，尽管有些葡萄球菌对甲氧西林耐药状态没有影响 观察到特定物种的影响，需要进一步调查。