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Dual-color STED super-resolution microscope using a single laser source.
Journal of Biophotonics ( IF 2.8 ) Pub Date : 2020-06-05 , DOI: 10.1002/jbio.202000057 Jialin Wang 1 , Jia Zhang 1 , Luwei Wang 1 , Xinwei Gao 1 , Yonghong Shao 1 , Liwei Liu 1 , Zhigang Yang 1 , Wei Yan 1 , Junle Qu 1
Journal of Biophotonics ( IF 2.8 ) Pub Date : 2020-06-05 , DOI: 10.1002/jbio.202000057 Jialin Wang 1 , Jia Zhang 1 , Luwei Wang 1 , Xinwei Gao 1 , Yonghong Shao 1 , Liwei Liu 1 , Zhigang Yang 1 , Wei Yan 1 , Junle Qu 1
Affiliation
STED (stimulated emission depletion) microscopy is one of the most promising super‐resolution fluorescence microscopies,due to its fast imaging and ultra‐high resolution. In this paper, we present a dual‐color STED microscope with a single laser source. Polarization beam splitters are used to separate the output from a supercontinuum laser source into four laser beams, including two excitation beams (488, 635 nm) and two depletion beams (592, 775 nm). These four laser beams are then used to build a low cost dual‐color STED system to achieve a spatial resolution of 75 nm in cell samples.
中文翻译:
使用单个激光源的双色STED超分辨率显微镜。
由于其快速成像和超高分辨率,STED(受激发射损耗)显微镜是最有前途的超分辨率荧光显微镜检查之一。在本文中,我们介绍了具有单个激光源的双色STED显微镜。偏振分束器用于将超连续谱激光源的输出分成四个激光束,包括两个激发光束(488、635 nm)和两个耗尽光束(592、775 nm)。然后,使用这四个激光束构建低成本的双色STED系统,以在细胞样本中实现75 nm的空间分辨率。
更新日期:2020-06-05
中文翻译:
使用单个激光源的双色STED超分辨率显微镜。
由于其快速成像和超高分辨率,STED(受激发射损耗)显微镜是最有前途的超分辨率荧光显微镜检查之一。在本文中,我们介绍了具有单个激光源的双色STED显微镜。偏振分束器用于将超连续谱激光源的输出分成四个激光束,包括两个激发光束(488、635 nm)和两个耗尽光束(592、775 nm)。然后,使用这四个激光束构建低成本的双色STED系统,以在细胞样本中实现75 nm的空间分辨率。