The use of biopolymers has gained priority in tissue engineering and biotechnology, both as dressing material and for enhancing treatment efficiency. There is a demand for new biopolymers designed with protease inhibitors and antimicrobials. LL‐37 is an important antimicrobial peptide in human skin and exhibits a broad spectrum of antimicrobial activity against bacteria, fungi, and viral pathogens. Using lignin which is an abundant carbohydrate polymer in nature and a polyacrylic acid, we prepared a lignin/caprolactone biodegradable film by plastifying caprolactone and polyacyrlic acid. Lignin/caprolactone biodegradable film was activated with CDI and then immobilized LL‐37 peptide. The structure was elucidated in terms of its functional groups by attenuated total reflectance‐fourier transform infrared spectroscopy (ATR‐FTIR), and the morphology of the lignin/caprolactone biodegradable film was characterized by scanning electron microscopy (SEM) before and after the immobilization process. The amount of LL‐37 immobilized was determined by ELISA method. It was found that 97% of LL‐37 peptide was successfully immobilized onto the lignin/caprolactone biodegradable film. Antimicrobial activity was determined in the lignin/caprolactone biodegradable film samples by quantitative antimicrobial activity method. According to the results, LL‐37 immobilized lignin/caprolactone biodegradable film samples were effective on test organisms; Gram‐positive Staphylococcus aureus and Gram‐negative Escherichia coli. In bio‐compatibility assays, the ability to support tissue cell integration was detected by using 3 T3 mouse fibroblasts. Samples were examined under transverse microscope, non‐immobilized sample showed a huge cellular death, whereas LL‐37 immobilized lignin/caprolactone biodegradable film had identical cellular growth with the control group. This dual functional lignin/caprolactone biodegradable film with enhanced antibacterial properties and increased tissue cell compatibility may be used to design new materials for various types of biological applications.

中文翻译：

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LL-37肽固定化木质素/己内酯聚合物薄膜的制备及抗菌性能

生物聚合物的使用已在组织工程和生物技术中得到优先考虑，既可以作为敷料，又可以提高治疗效率。需要设计有蛋白酶抑制剂和抗微生物剂的新型生物聚合物。LL-37是人类皮肤中重要的抗菌肽，对细菌，真菌和病毒病原体具有广泛的抗菌活性。我们使用自然界中丰富的碳水化合物聚合物木质素和聚丙烯酸，通过将己内酯和聚丙烯酸增塑来制备木质素/己内酯可生物降解膜。木质素/己内酯可生物降解膜被CDI激活，然后被固定化LL-37肽。通过衰减全反射-傅立叶变换红外光谱（ATR-FTIR）阐明了其功能基团的结构，木质素/己内酯生物降解膜的形貌通过固定化前后的扫描电镜观察。固定化LL-37的量通过ELISA方法确定。发现97％的LL-37肽已成功固定在木质素/己内酯可生物降解膜上。通过定量抗菌活性方法测定木质素/己内酯可生物降解薄膜样品中的抗菌活性。根据结果​​，固定化LL-37木质素/己内酯的可生物降解薄膜样品对测试生物有效。革兰氏阳性 发现97％的LL-37肽已成功固定在木质素/己内酯可生物降解膜上。通过定量抗菌活性方法测定木质素/己内酯可生物降解薄膜样品中的抗菌活性。根据结果​​，固定化LL-37木质素/己内酯的可生物降解薄膜样品对测试生物有效。革兰氏阳性 发现97％的LL-37肽已成功固定在木质素/己内酯可生物降解膜上。通过定量抗菌活性方法测定木质素/己内酯可生物降解薄膜样品中的抗菌活性。根据结果​​，固定化LL-37木质素/己内酯的可生物降解薄膜样品对测试生物有效。革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌。在生物相容性测定中，通过使用3个T3小鼠成纤维细胞检测了支持组织细胞整合的能力。在横向显微镜下检查样品，未固定的样品显示出巨大的细胞死亡，而固定有LL-37的木质素/己内酯可生物降解膜的细胞生长与对照组相同。这种具有增强的抗菌性能和增强的组织细胞相容性的双功能木质素/己内酯生物可降解薄膜可用于设计用于各种类型生物应用的新材料。