Researches of biomaterials for osteoporotic bone defects focus on the improvement of its anti‐osteoporosis ability, due to osteoporosis is a kind of systemic and long‐range bone metabolism disorder. Nevertheless, how to steadily deliver anti‐osteoporosis drugs in osteoporotic bone defects is rarely studied. Reported evidences have shown that alendronate (Aln) is known to not only restrain osteoclasts from mediating bone resorption but also stimulate osteoblasts to regenerate bone tissue. Here, we developed an engineered implantable scaffold that could sustainably release Aln for osteoporotic bone defects. Briefly, Aln was added into 2% collagen (Col) solution to form a 5 mg/ml mixture. Then the mixture was filled into pre‐designed round models (diameter: 5 mm, height: 2 mm) and crosslinked to obtain engineered Col‐Aln scaffolds. The release kinetics showed that Aln was released at an average rate of 2.99 μg/d in the initial 8 days and could sustainably release for 1 month. To detect the repair effects of the Col‐Aln scaffolds for osteoporotic defects, the Col and Col‐Aln scaffolds were implanted into 5 mm cranial defects in ovariectomized rats. After 3 months, the cranial defects implanted with Col‐Aln scaffolds achieved more bone regeneration in defect area (11.74 ± 3.82%) than Col scaffold (5.12 ± 1.15%) (p < .05). Moreover, ovariectomized rats in Col‐Aln scaffold group possessed more trabecular bone in femur metaphysis than Col scaffold group as analyzed by Micro‐CT. This study demonstrated the engineered Col‐Aln scaffold has the potential to repair osteoporotic bone defects and resist bone loss in osteoporosis.

中文翻译：

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通过工程胶原支架持续递送阿仑膦酸钠，用于修复骨质疏松性骨缺损和抵抗骨质流失。

骨质疏松性骨缺损生物材料的研究主要集中在提高其抗骨质疏松能力，因为骨质疏松是一种全身性、长程性骨代谢紊乱。然而，很少研究如何在骨质疏松性骨缺损中稳定输送抗骨质疏松药物。已报道的证据表明，已知阿仑膦酸盐 (Aln) 不仅可以抑制破骨细胞介导骨吸收，还可以刺激成骨细胞再生骨组织。在这里，我们开发了一种工程植入式支架，可以持续释放 Aln 以治疗骨质疏松性骨缺损。简而言之，将 Aln 添加到 2% 胶原 (Col) 溶液中以形成 5 mg/ml 的混合物。然后将混合物填充到预先设计的圆形模型（直径：5 毫米，高度：2 毫米）中并交联以获得工程化的 Col-Aln 支架。释放动力学表明，Aln在最初8天的平均释放速率为2.99 μg/d，可持续释放1个月。为了检测 Col-Aln 支架对骨质疏松性缺损的修复效果，将 Col 和 Col-Aln 支架植入去卵巢大鼠的 5 mm 颅骨缺损处。3个月后，植入Col-Aln支架的颅骨缺损区域（11.74±3.82%）比Col支架（5.12±1.15%）实现了更多的骨再生（p  < .05）。此外，通过 Micro-CT 分析，Col-Aln 支架组切除卵巢的大鼠股骨干骺端具有比 Col 支架组更多的小梁骨。这项研究表明，工程化的 Col-Aln 支架具有修复骨质疏松性骨缺损和抵抗骨质疏松症骨质流失的潜力。