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Caspase-mediated cleavage of nucleocapsid protein of a protease-independent porcine epidemic diarrhea virus strain.
Virus Research ( IF 5 ) Pub Date : 2020-05-18 , DOI: 10.1016/j.virusres.2020.198026
Changin Oh 1 , Yunjeong Kim 1 , Kyeong-Ok Chang 1
Affiliation  

Porcine epidemic diarrhea virus (PEDV) infection in neonatal piglets can cause up to 100% mortality, resulting in significant economic loss in the swine industry. Like other coronaviruses, PEDV N protein is a nucleocapsid protein and abundantly presents at all stages of infection. Previously, we reported that the N protein of trypsin-independent PEDV 8aa is cleaved during virus replication. In this study, we further investigated the nature of N protein cleavage using various methods including protease cleavage assays with or without various inhibitors and mutagenesis study. We found that PEDV 8aa infection in Vero cells leads to apoptotic cell death, and caspase 6 or 7 can cleave PEDV 8aa N protein at the late stage of the replication. The caspase-mediated cleavage occurs between D424 and G425 near the C-terminal of N protein. We also report that both cleaved and uncleaved N proteins are exclusively localized in the cytoplasm of PEDV infected cells.

中文翻译:

不依赖蛋白酶的猪流行性腹泻病毒株的 Caspase 介导的核衣壳蛋白裂解。

新生仔猪感染猪流行性腹泻病毒 (PEDV) 可导致高达 100% 的死亡率,从而导致养猪业的重大经济损失。与其他冠状病毒一样,PEDV N 蛋白是一种核衣壳蛋白,大量存在于感染的各个阶段。以前,我们报道了不依赖胰蛋白酶的 PEDV 8aa 的 N 蛋白在病毒复制过程中被切割。在这项研究中,我们使用各种方法进一步研究了 N 蛋白裂解的性质,包括使用或不使用各种抑制剂的蛋白酶裂解测定和诱变研究。我们发现 Vero 细胞中的 PEDV 8aa 感染导致细胞凋亡,半胱天冬酶 6 或 7 可以在复制后期切割 PEDV 8aa N 蛋白。Caspase 介导的切割发生在 D424 和 G425 之间 N 蛋白 C 端附近。
更新日期:2020-05-18
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