Our main study objective was to determine the prevalence and trend of parasitic infection in client-owned dogs examined at the veterinary parasitology diagnostic laboratory of Oklahoma State University over the past 12 years. All results of centrifugal flotation, saline direct smear, sedimentation, Baermann, acid-fast staining for Cryptosporidium detection, and Giardia antigen examinations on fecal samples from client-owned dogs submitted to the Boren Veterinary Medical Hospital and Oklahoma Animal Disease Diagnostic Laboratory of Oklahoma State University from 2007 through 2018 were included. The impact of sex, age, and seasonality on the prevalence of parasitic infection was also statistically evaluated. A total of 7,409 cases were included for this study. Majority of cases (79.58%; 5,896/7,409) did not include any parasites, eggs, larva, oocysts, or cysts. Approximately 15.41% (1,142/7,409) of client-owned dogs were infected by at least one parasite, and 5.01% (371/7,409) of dogs were infected by multiple parasites. The most common parasite stage observed was Ancylostoma eggs (8.23%; 610/7,409), followed by Cystoisospora oocysts (5.02%; 372/7,409), Giardia cysts/antigen (4.06%; 301/7,409), Trichuris vulpis eggs (2.74%; 203/7,409), Toxocara canis eggs (2.54%; 188/7,409), Dipylidium caninum proglottids/egg packets (0.84%; 62/7,409), taeniid proglottids/eggs (0.47%; 35/7,409), Sarcocystis sporocysts (0.38%; 28/7,409), Cryptosporidium oocysts (0.30%; 22/7,409), Strongyloides stercoralis larvae (0.20%; 15/7,409), Alaria eggs (0.19%; 14/7,409), Toxascaris leonina eggs (0.18%; 13/7,409), Capillaria eggs (0.16%; 12/7,409), Hammondia-like small coccidian oocysts (0.16%; 12/7,409), Uncinaria stenocephala eggs (0.13%; 10/7,409), Spirometra eggs (0.09%; 7/7,409), Physaloptera eggs (0.09%; 7/7,409), Heterobilharzia americana eggs (0.08%; 6/7,409), Nanophyetus salmincola eggs (0.08%; 6/7,409), trichomonads (0.08%; 6/7,409), Mesocestoides proglottids/eggs (0.05%; 4/7,409), Baylisascaris eggs (0.01%; 1/7,409), Macracanthorhynchus eggs (0.01%; 1/7,409), and Paragonimus kellicotti eggs (0.01%; 1/7,409). In addition to endoparasites, some ectoparasites, such as Demodex mites (0.22%; 16/7,409), Otodectes cynotis mites (0.01%; 1/7,409), Rhipicephalus sanguineus ticks (0.01%; 1/7,409), and Sarcoptes scabiei mites (0.01%; 1/7,409), were detected by fecal examinations. Pseudo/spurious parasites were identified in approximately 4.35% of cases (322/7,409). There was no statistically significant difference for parasite prevalence between sexes (p = 0.3231). However, statistically significant differences were observed with certain parasites when compared by age groups, and generally, prevalence of parasitism decreased as age of client-owned dogs increased (p < 0.0001). Statistical analyses also revealed significant differences by months (p = 0.0013). Overall, the prevalence of parasitic infection in client-owned dogs decreased over the past 12 years (p < 0.0001).

我们的主要研究目标是确定过去12年间在俄克拉荷马州立大学兽医寄生虫学诊断实验室检查的客户拥有的狗的寄生虫感染的流行率和趋势。离心浮选，盐水直接涂片，沉淀，Baermann，用于隐孢子虫检测的耐酸染色和贾第虫的所有结果包括从2007年至2018年提交给俄克拉荷马州立大学Boren兽医医院和俄克拉荷马州动物疾病诊断实验室的客户拥有的狗的粪便样本的抗原检查。性别，年龄和季节性对寄生虫感染发生率的影响也进行了统计学评估。本研究共纳入7,409例。大多数病例（79.58％； 5,896 / 7,409）不包括任何寄生虫，卵，幼虫，卵囊或囊肿。约有15.41％（1,142 / 7,409）的客户拥有的狗被至少一种寄生虫感染，而5.01％（371 / 7,409）的狗被多种寄生虫感染。观察到的最常见的寄生虫阶段是Ancylostoma卵（8.23％； 610 / 7,409），其次是Cysoisosospora卵囊（5.02％; 372/7409），贾第虫属孢囊/抗原（4.06％; 301/7409），鞭vulpis蛋（2.74％; 203/7409），犬弓首蛔虫蛋（2.54％; 188/7409），犬复孔绦虫节片/蛋分组（0.84％; 62/7409），taeniid节片/蛋（0.47％; 35/7409），肉孢子虫孢子囊（0.38％; 28/7409），隐孢子虫的卵囊（0.30％; 22/7409），粪类圆线虫的幼虫（0.20％; 15/7409），Alaria蛋（0.19％; 14/7409），Toxascaris Leonina酒店鸡蛋（0.18％; 13/7409），毛细线虫蛋（0.16％; 12/7409），Hammondia样小球虫卵囊（0.16％; 12/7409），钩虫stenocephala鸡蛋（0.13％; 10/7409），绦虫卵（0.09％; 7/7409），泡翼蛋（0.09％; 7/7409），Heterobilharzia美洲鸡蛋（0.08％; 6/7409），Nanophyetus salmincola蛋（0.08％; 6/7409），毛滴虫（0.08％; 6/7409），Mesocestoides节片/蛋（0.05％; 4/7409），Baylisascaris鸡蛋（0.01％ ； 1 / 7,409），Macracanthorhynchus卵（0.01％； 1 / 7,409）和Paragonimus kellicotti卵（0.01％； 1 / 7,409）。除了体内寄生虫，还有一些体外寄生虫，例如蠕形螨（Demodex螨）（0.22％; 16 / 7,409），Otodectes cynotis螨（0.01％; 1/7409），血红扇头蜱蜱（0.01％; 1/7409），和疥螨（0.01％; 1/7409）中，通过粪便检查检测。在大约4.35％的病例中（322 / 7,409）鉴定出伪/寄生虫。男女之间的寄生虫流行率无统计学差异（p = 0.3231）。但是，按年龄组比较，某些寄生虫在统计学上有显着差异，并且通常，寄生虫的患病率随客户拥有的狗的年龄增加而降低（p <0.0001）。统计分析还显示，每月之间存在显着差异（p= 0.0013）。总体而言，在过去的12年中，客户拥有的狗中的寄生虫感染发生率有所下降（p <0.0001）。