This study aims to inspect the effects of Imidacloprid (IMI) acute toxicity in the ovaries of the red palm weevil (Rhynchophorus ferrugineus). The LC₅₀ of IMI was determined to be 15.7 ppm. We assessed the biochemical changes resulting from treatment with four IMI concentrations (10, 15, 20 and 30 ppm). IMI reduced acetylcholine esterase activity in a dose-dependent manner. Glutathione (GSH) content decreased with increasing concentration of IMI; however, GSH was not affected and accompanied by an increase in catalase activity with the highest concentration of IMI. Lipid peroxidation increased steadily as IMI concentration increased. Based on the ultrastructural analyses of the follicle cells and the ooplasm of developing oocytes, acute IMI toxicity produced damaged yolk bodies, pale nuclei, and shrinking microvilli. Fractionation of rough endoplasmic reticulum, deformed mitochondria, and autophagic bodies were also observed. In conclusion, these ovarian pathobiochemical changes could be used as biomarkers of acute IMI exposure.

这项研究旨在检查吡虫啉（IMI）对红色棕榈象鼻虫（Rhynchophorus ferrugineus）卵巢的急性毒性作用。LC ₅₀测定的IMI为15.7ppm。我们评估了用四种IMI浓度（10、15、20和30 ppm）处理产生的生化变化。IMI以剂量依赖性方式降低了乙酰胆碱酯酶的活性。谷胱甘肽（GSH）含量随IMI浓度的增加而降低；然而，谷胱甘肽没有受到影响，并伴随着IMI浓度最高的过氧化氢酶活性的增加。脂质过氧化随着IMI浓度的增加而稳定增加。根据卵泡细胞和发育中的卵母细胞的卵泡的超微结构分析，急性IMI毒性可产生卵黄质受损，核淡，微绒毛萎缩。还观察到粗糙的内质网，变形的线粒体和自噬体的分离。结论，