Aphidius gifuensis is an important biological control agent of aphid. It stores sugar as energy reserves before the onset of diapause, a form of dormancy that occurs when environmental conditions become unfavorable for development. In this study, cDNA of three relevant enzymes, trehalose-6-phosphate synthase (TPS), glycogen synthase (GYS) and glycogen phosphorylase (GP) were cloned, and their expression profiles in the preparation and maintenance of diapause, and post-diapause development were analyzed. The results showed that AgTPS, AgGYS and AgGP encoded proteins consisting of 807, 690 and 844 amino acids respectively. The expression profiles of three genes were significantly affected by diapause-inducing condition. The increase of transcription of AgGYS and AgGP were followed by the up-regulate of AgTPS expression in the preparation of diapause, expression of all three genes were inhibited while diapause is maintained, and expression of AgGYS was up-regulated when diapause was terminated. These results suggested that trehalose is accumulated before initiation of diapause and glycogen may play an important role in post-diapause development.

岐阜蚜虫是蚜虫的重要生物防治剂。它在滞育开始之前将糖存储为能量储备，滞育是在环境条件变得不利于发展时发生的一种休眠形式。在这项研究中，克隆了三种相关酶的cDNA，即海藻糖6-磷酸合酶（TPS），糖原合酶（GYS）和糖原磷酸化酶（GP），并且它们在滞育和维持以及滞后后的表达谱发展进行了分析。结果表明，AgTPS，AgGYS和AgGP分别编码由807、690和844个氨基酸组成的蛋白质。滞育诱导条件显着影响了三个基因的表达谱。AgGYS的转录增加和AgGP被随后上调AgTPS中滞育的制备表达，所有这三个基因的表达受到抑制而滞育被维持，并且表达AgGYS上调时滞育被终止。这些结果表明海藻糖在滞育前开始积累，糖原可能在滞后发展中起重要作用。