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The efficient induction of tetraploid plants via adventitious shoots in Antirrhinum majus L. by in vitro seed treatment with amiprophos-methyl
Plant Cell, Tissue and Organ Culture ( IF 3 ) Pub Date : 2020-05-18 , DOI: 10.1007/s11240-020-01849-8
The Su Hlaing , H. Kondo , A. Deguchi , K. Miyoshi

Tetraploid plants of Antirrhinum majus L. were obtained from the seeds of the F1 variety ‘Maryland True Pink’ by amiprophos-methyl (APM) treatment and subsequent in vitro culture. Seeds were treated with 0, 10 and 20 mgl−1 APM for 1, 3, or 7 d (day) with or without osmopriming. Overall, the seedlings established from the seeds treated with 10 or 20 mgl−1 APM for 3–7 d exhibited malformation with rudimentary roots and swollen hypocotyls that eventually produced adventitious shoots. The highest adventitious shoot formation frequency reached 71.6% in non-primed seeds treated with 10 mgl−1 APM for 7 d; this decreased to 54.4% in osmoprimed seeds that received the same APM treatment. The plantlets from the adventitious shoots were identified as tetraploids at frequencies of 51.4% and 90.4%, respectively. Tetraploid frequency increased with higher concentration and/or longer duration of APM treatment and ranged from 0 to 65.9% in the non-primed seeds and 10.3% to 100% in the osmoprimed seeds. Osmopriming of seeds accelerated the induction of tetraploids by APM treatment at the same concentration and duration by 10.3–39.0%, except in the case of treatment with 20 mgl−1 APM for 3 d treatment. In the osmoprimed seeds treated with 20 mgl−1 APM for 7 d, all the plantlets from the adventitious shoots were revealed to be tetraploids. This study revealed that APM has dual effects, namely the inhibitory effect on the growth of seedlings and the stimulatory effect on the induction of adventitious shoots on the hypocotyl of seedlings.



中文翻译:

rrh虫甲基种子体外处理通过不定芽有效诱导四倍体植物

的四倍体植株金鱼草从的F中的种子获得L. 1品种“马里兰真粉红”通过amiprophos甲基(APM)处理和在体外培养之后。将种子用0、10和20 mgl -1 APM处理1、3或7天(天),并进行或不进行渗透压处理。总体而言,用10或20 mgl -1 APM处理3–7 d的种子所形成的幼苗表现出畸形,具有根粗和下胚轴肿胀,最终产生不定芽。在10 mgl -1处理的未上底漆种子中,最高不定芽形成频率达到71.6%APM 7天;在接受相同APM处理的渗透底种子中,这一比例降至54.4%。来自不定芽的小苗被鉴定为四倍体,频率分别为51.4%和90.4%。四倍体频率随着APM处理浓度的增加和/或持续时间的延长而增加,在非底漆种子中从0到65.9%,在渗透底漆种子中从10.3%到100%。种子的渗透处理在相同浓度和持续时间内通过APM处理加速了四倍体的诱导,增加了10.3–39.0%,除非在20 mgl -1 APM处理3 d的情况下。在用20 mgl -1处理的渗透启动种子中APM 7天,所有不定芽的幼苗均显示为四倍体。这项研究表明,APM具有双重作用,即对幼苗生长的抑制作用和对幼苗下胚轴不定芽诱导的刺激作用。

更新日期:2020-05-18
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