Bacillus species are promising agents for the biological control of postharvest diseases. Based on the morphological, physiological and biochemical characteristics and 16S rRNA gene sequence analysis, the bacterial strain J-1 was identified as Bacillus atrophaeus. The B. atrophaeus strain J-1 showed broad spectrum antifungal activity against various plant pathogenic fungi. In assays in vitro the metabolites contained in the B. atrophaeus strain J-1 cell-free filtrate had a strong inhibitory effect on Botryosphaeria dothidea. The cell-free filtrate inhibited the growth of B. dothidea in vivo and reduced the disease incidence. Furthermore, PCR detection showed that the strain J-1 had lipopeptide antibiotic biosynthesis genes: fengycin, iturin A and surfactin. The lipopeptide crude extractions from the strain J-1 cell-free filtrate significantly suppressed the growth of B. dothidea. C14 iturin A was isolated by bioactivity-guided fractionation and identified by liquid chromatography–electronic spray ionization–tandem mass spectrometry analysis. This paper is the first to report that B. atrophaeus J-1 efficiently controls apple ring rot disease caused by B. dothidea. Using the B. atrophaeus strain J-1 may provide an alternative control method for the prevention apple ring rot disease.

中文翻译：

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萎缩芽孢杆菌J-1菌株作为苹果环腐病生物制剂的鉴定与表征

芽孢杆菌种是用于收获后疾病的生物防治的有前途的试剂。根据形态，生理生化特性和16S rRNA基因序列分析，确定细菌菌株J-1为萎缩芽孢杆菌。所述B.萎缩菌株J-1显示出对各种植物致病真菌的广谱抗真菌活性。在体外测定中包含的代谢物B.萎缩J-1菌株的无细胞滤液对较强的抑制作用杨树dothidea侵染。无细胞滤液抑制了B. dothidea的生长在体内减少了疾病的发生。此外，PCR检测表明，菌株J-1具有脂肽抗生素生物合成基因：风霉素，伊图林A和表面活性素。从菌株J-1无细胞滤液中提取的脂肽粗提取物显着抑制了B. dothidea的生长。通过生物活性指导分离分离C14 iturin A，并通过液相色谱-电子喷雾电离-串联质谱分析进行鉴定。本文是第一篇报道萎缩双歧杆菌J-1有效控制由多形双歧杆菌引起的苹果环腐病的报道。使用萎缩双歧杆菌菌株J-1可以为预防苹果环腐病提供另一种控制方法。