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Development of a real-time loop-mediated isothermal amplification method for the detection of severe fever with thrombocytopenia syndrome virus.
Journal of Microbiology ( IF 3 ) Pub Date : 2020-05-18 , DOI: 10.1007/s12275-020-0109-1
Jae Woong Lee 1 , Yu-Jung Won 1 , Lae Hyung Kang 1 , Sung-Geun Lee 2 , Seung-Won Park 3 , Soon-Young Paik 1
Affiliation  

Severe fever with thrombocytopenia syndrome (SFTS) is being reported annually in South Korea since its first detection there in 2010. The causal agent is a negative-strand RNA virus 80–100 nm in diameter. It causes fever, thrombocytopenia, leukocytopenia, gastrointestinal symptoms, and neural symptoms. The mortality rate of SFTS was 32.6% among 172 cases reported from 2012 to 2015 in South Korea. Thus, is necessary to develop an effective diagnostic method that selectively identifies the isolates circulating in South Korea. The real-time reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay is a simple, rapid, and sensitive approach for molecular diagnosis. Here, we designed novel primers for this assay and found that the technique had very high specificity, sensitivity, and efficiency. This real-time RT-LAMP approach using the novel primers developed herein can be applied for early diagnosis of SFTSV strains in South Korea to reduce the mortality rate of SFTS.

中文翻译:

实时循环介导的等温扩增方法的开发,用于检测血小板减少症候群病毒的严重发热。

自2010年在韩国首次发现以来,每年都报告有血小板减少综合征(SFTS)的严重发烧。病原体是直径80-100 nm的负链RNA病毒。它会引起发烧,血小板减少,白细胞减少,胃肠道症状和神经症状。在2012年至2015年韩国报告的172例病例中,SFTS的死亡率为32.6%。因此,有必要开发一种有效的诊断方法,以选择性地鉴定在韩国流通的分离株。实时逆转录环介导的等温扩增(RT-LAMP)分析是一种简单,快速且灵敏的分子诊断方法。在这里,我们设计了用于该测定的新型引物,发现该技术具有很高的特异性,灵敏度和效率。
更新日期:2020-05-18
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