In continuation of our work on Tagetes spp., the current study deals with the nematicidal activity of flavonoids, which are structurally related to two marker compounds, patuletin and patulitrin isolated from Tagetes patula flowers. The activity was determined against root-knot nematode, Meloidogyne incognita to establish structure activity relationship. In vitro examination of flavonoids belonging to flavone and flavonol classes revealed significant differences in nematicidal ability depending on their structural motif, concentration, and time of exposure. In case of flavonols the most efficient compounds were found to be fisetin, galangin, isorhamnetin, morin, and myricetin which exhibited 100% mortality of 2nd stage juveniles after 24 h of incubation. Whereas, among flavones which showed the same excellent activity parameters, were simple flavone, 6-hydroxy flavone, chrysin, 6-methoxy apigenin, apigenin-7-O-glucoside and 6-methoxy luteolin. These results revealed that flavonoids possess promising nematicidal activity and may have potential use in crop management as active agents.

在继续我们对Tagetes spp。的研究中，当前的研究涉及类黄酮的杀线虫活性，该类黄酮的结构与从Tagetes patula花中分离出的两种标记化合物patuletin和patulitrin有关。确定了针对根结线虫Meloidogyne incognita的活性建立结构活动关系。属于黄酮类和黄酮类的类黄酮的体外检查显示，根据其结构图案，浓度和暴露时间，杀线虫能力存在显着差异。对于黄酮醇，发现最有效的化合物是非瑟定，高良姜精，异鼠李素，莫林和杨梅素，它们在孵育24小时后表现出100％的第二阶段幼虫死亡率。而在显示出相同优异活性参数的黄酮中，简单的黄酮，6-羟基黄酮，菊花，6-甲氧基芹菜素，芹菜素-7- O-葡糖苷和6-甲氧基木犀草素。这些结果表明，类黄酮具有良好的杀线虫活性，并可能在作物管理中作为活性剂使用。