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Mechanisms of Acinetobacter baumannii Capsular Polysaccharide Cleavage by Phage Depolymerases
Biochemistry (Moscow) ( IF 2.8 ) Pub Date : 2020-05-01 , DOI: 10.1134/s0006297920050053
Y A Knirel 1 , M M Shneider 2, 3 , A V Popova 3, 4, 5 , A A Kasimova 1, 6 , S N Senchenkova 1 , A S Shashkov 1 , A O Chizhov 1
Affiliation  

Aerobic gram-negative bacterium Acinetobacter baumannii has recently become one of the most relevant pathogens associated with hospital-acquired infections worldwide. A. baumannii produces a capsule around the cell, which represents a thick viscous layer of structurally variable capsular polysaccharide (CPS). The capsule protects the bacteria against unfavorable environmental factors and biological systems, including bacteriophages and host immune system. Many A. baumannii phages have structural depolymerases (tailspikes) that specifically recognize and digest bacterial CPS. In this work, we studied the interaction of tailspike proteins of four lytic depolymerase-carrying phages with A. baumannii CPS. Depolymerases of three bacteriophages (Fri1, AS12, and BS46) were identified as specific glycosidases that cleave the CPS of A. baumannii strains 28, 1432, and B05, respectively, by the hydrolytic mechanism. The gp54 depolymerase from bacteriophage AP22 was characterized as a polysaccharide lyase that cleaves the CPS of A. baumannii strain 1053 by β-elimination at hexuronic acid (ManNAcA) residues.

中文翻译:

噬菌体解聚酶裂解鲍曼不动杆菌荚膜多糖的机制

需氧革兰氏阴性菌鲍曼不动杆菌最近已成为世界范围内与医院获得性感染相关的最相关病原体之一。鲍曼不动杆菌在细胞周围产生一个荚膜,它代表结构可变的荚膜多糖 (CPS) 的厚粘性层。胶囊保护细菌免受不利的环境因素和生物系统的影响,包括噬菌体和宿主免疫系统。许多鲍曼不动杆菌噬菌体具有结构解聚酶(尾刺),可特异性识别和消化细菌 CPS。在这项工作中,我们研究了四种携带裂解解聚酶的噬菌体的尾刺蛋白与鲍曼不动杆菌 CPS 的相互作用。三种噬菌体(Fri1、AS12 和 BS46)的解聚酶被鉴定为特异性糖苷酶,可切割鲍曼不动杆菌菌株 28 的 CPS,1432 和 B05 分别通过水解机制。来自噬菌体 AP22 的 gp54 解聚酶被表征为一种多糖裂解酶,它通过β-消除己糖醛酸 (ManNAcA) 残基来切割鲍曼不动杆菌 1053 菌株的 CPS。
更新日期:2020-05-01
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