Like B cells, T cells can be immortalized through hybridization with lymphoma cells, a technique that has been particularly useful in the study of the T cell receptors (TCR) for antigen. In T cell hybridizations, the AKR mouse strain-derived thymus lymphoma BW5147 is by far the most popular fusion line. However, the full potential of this technology had to await inactivation of the productively rearranged TCR-α and -β genes in the lymphoma. BWα-β-, the TCR-gene deficient variant of the original lymphoma, which has become the fusion line of choice for αβ T cells, is now available with numerous modifications, enabling the investigation of many aspects of TCR-mediated responses and TCR-structure. Unexpectedly, inactivating BW's functional TCR-α gene also rendered the lymphoma more permissive for the expression of TCR-γδ, facilitating the study of γδ T cells, their TCRs, and their TCR-mediated reactivity.

中文翻译：

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BW5147及其衍生物，用于研究T细胞及其抗原受体。

像B细胞一样，T细胞可通过与淋巴瘤细胞杂交而永生化，该技术在研究抗原的T细胞受体（TCR）中特别有用。在T细胞杂交中，AKR小鼠品系的胸腺淋巴瘤BW5147是迄今为止最受欢迎的融合株。但是，这项技术的全部潜力必须等待淋巴瘤中生产性重排的TCR-α和-β基因失活。BWα-β-是原始淋巴瘤的TCR基因缺陷型变体，现已成为αβT细胞的选择融合线，现已经过大量修饰，可用于研究TCR介导的反应和TCR-结构体。出乎意料的是，BW的功能性TCR-α基因失活也使淋巴瘤更容易表达TCR-γδ，