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Sub-lethal insecticide exposure affects host biting efficiency of Kdr-resistant Anopheles gambiae
bioRxiv - Animal Behavior and Cognition Pub Date : 2020-07-01 , DOI: 10.1101/653980
Malal M Diop , Fabrice Chandre , Marie Rossignol , Angélique Porciani , Mathieu Chateau , Nicolas Moiroux , Cédric Pennetier

The massive use of insecticide-treated nets (ITNs) has drastically changed the environment for malaria vector mosquitoes, challenging their host-seeking behaviour and biting success. Here, we investigated the effect of a brief exposure to an ITN on the biting behaviour of Anopheles gambiae mosquitoes and the interaction between such behaviour and the kdr mutation that confers resistance to pyrethroids. To this aim, we developed a video assay to study the biting behaviour of mosquitoes with similar genetic background, but different kdr locus genotypes (SS i.e. homozygous susceptible, RS i.e. heterozygous and RR i.e. homozygous resistant), after a brief exposure to either control untreated nets or one of two types of pyrethroid-treated nets (deltamethrin or permethrin). In presence of untreated nets, the kdr mutation did not influence mosquito blood feeding success but caused differences in feeding and prediuresis durations and blood meal size. Exposure to deltamethrin ITN decreased the blood feeding success rate of RR and RS mosquitoes, whereas in presence of permethrin ITN, the kdr mutation increased the blood-feeding success of mosquitoes. Exposure to the two types of pyrethroid-treated nets reduced feeding duration, prediuresis duration and blood meal size of all three genotypes. Our study demonstrates a complex interaction between insecticide exposure and the kdr mutation on the biting behavior of mosquitoes, which may substantially impact malaria vector fitness and disease transmission.

中文翻译:

亚致死性杀虫剂暴露影响耐Kdr冈比亚按蚊的宿主咬食效率

大量使用经杀虫剂处理的蚊帐(ITN)极大地改变了疟疾媒介蚊子的环境,挑战了它们寻找宿主的行为并取得了成功。在这里,我们调查了短暂暴露于ITN对冈比亚按蚊蚊咬的行为以及这种行为与赋予拟除虫菊酯抗性的kdr突变之间相互作用的影响。为了这个目标,我们开发了一个视频实验来研究具有相似遗传背景但不同kdr位点基因型(SS即纯合易感性,RS即杂合性和RR即纯合性抗性)的蚊子在短暂暴露于未处理的任何对照后的咬食行为蚊帐或拟除虫菊酯处理过的两种蚊帐之一(溴氰菊酯或苄氯菊酯)。在未经处理的蚊帐的情况下,kdr突变不会影响蚊子的采血成功率,但会导致采食和利尿持续时间以及血粉量的差异。溴氰菊酯ITN暴露会降低RR和RS蚊子的采血成功率,而氯菊酯ITN存在时,kdr突变会增加蚊子的采血成功率。暴露于两种拟除虫菊酯处理的蚊帐可缩短所有三种基因型的进食时间,利尿前时间和血粉量。我们的研究表明,杀虫剂暴露与kdr突变对蚊子叮咬行为之间存在复杂的相互作用,这可能会严重影响疟疾媒介的适应性和疾病传播。溴氰菊酯ITN暴露会降低RR和RS蚊子的采血成功率,而氯菊酯ITN存在时,kdr突变会增加蚊子的采血成功率。暴露于两种拟除虫菊酯处理过的蚊帐可缩短所有三种基因型的进食时间,利尿前时间和血粉量。我们的研究表明,杀虫剂暴露与kdr突变对蚊子叮咬行为之间存在复杂的相互作用,这可能会严重影响疟疾媒介的适应性和疾病传播。溴氰菊酯ITN暴露会降低RR和RS蚊子的采血成功率,而氯菊酯ITN存在时,kdr突变会增加蚊子的采血成功率。暴露于两种拟除虫菊酯处理过的蚊帐可缩短所有三种基因型的进食时间,利尿前时间和血粉量。我们的研究表明,杀虫剂暴露与kdr突变对蚊子叮咬行为之间存在复杂的相互作用,这可能会严重影响疟疾媒介的适应性和疾病传播。
更新日期:2020-07-01
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