Exposure to ambient air pollutants such as ozone (O3) and particulate matter (PM) is associated with increased cardiovascular morbidity and rate of mortality, but the underlying biological mechanisms have yet to be described. Emerging evidence shows that extracellular vehicle (EV) microRNAs (miRNAs) may facilitate cell-to-cell and organ-to-organ communications and play a role in the air pollution-induced cardiovascular effects. This study aims to explore the association between air pollutant exposure and miRNA changes related to cardiovascular diseases. Using a panel study design, 14 participants with coronary artery diseases were enrolled in this study. Each participant had up to 10 clinical visits and their plasma samples were collected and measured for expression of miRNA-21 (miR-21), miR-126, miR-146, miR-150, and miR-155. Mixed effects models adjusted for temperature, humidity, and season were used to examine the association between miRNA levels and exposure to 8-hr O3 or 24-hr PM2.5 up to 4 days prior. Results demonstrated that miR-150 expression was positively associated with O3 exposure at 1-4 days lag and 5day moving average while miR-155 expression tracked with O3 exposure at lag 0. No significant association was found between miRNA expression and ambient PM2.5 at any time point. β-blocker and diabetic medication usage significantly modified the correlation between O3 exposure and miR-150 expression where the link was more prominent among non-users. In conclusion, evidence indicated an association between exposure to ambient O3 and circulating levels of EV miR-150 and miR-155 was observed. These findings pointed to a future research direction involving miRNA-mediated mechanisms of O3-induced cardiovascular effects.

中文翻译：

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环境臭氧暴露对冠状动脉疾病患者循环细胞外载体微RNA水平的影响。

暴露于环境空气中的污染物，例如臭氧（O3）和颗粒物（PM），会增加心血管疾病的发病率和死亡率，但是尚未阐明其潜在的生物学机制。越来越多的证据表明，细胞外载体（EV）的microRNA（miRNA）可能促进细胞与细胞之间以及器官与器官之间的通讯，并在空气污染引起的心血管效应中发挥作用。这项研究旨在探讨空气污染物暴露与与心血管疾病相关的miRNA变化之间的关联。使用小组研究设计，招募了14位患有冠状动脉疾病的参与者。每个参与者最多进行10次临床就诊，收集其血浆样品并测量其miRNA-21（miR-21），miR-126，miR-146，miR-150和miR-155的表达。使用针对温度，湿度和季节进行了调整的混合效应模型，以检查miRNA水平与暴露于8天之前的8小时O3或24小时PM2.5之间的关联。结果表明，miR-150表达与1-4天滞后和5天移动平均值的O3暴露呈正相关，而miR-155表达与O3暴露的滞后0跟踪。任何时间点。β受体阻滞剂和糖尿病药物的使用显着改变了O3暴露与miR-150表达之间的相关性，其中非使用者之间的联系更为突出。总之，有证据表明，观察到环境O3暴露与EV miR-150和miR-155的循环水平之间存在关联。