Systematic Analysis of Kelch Repeat F-box (KFB) Protein Gene Family and Identification of Phenolic Acid Regulation Members in Salvia miltiorrhiza Bunge,Genes

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Systematic Analysis of Kelch Repeat F-box (KFB) Protein Gene Family and Identification of Phenolic Acid Regulation Members in Salvia miltiorrhiza Bunge
Genes ( IF 3.5 ) Pub Date : 2020-05-16 , DOI: 10.3390/genes11050557
Haizheng Yu _{1,

2} , Mengdan Jiang ₃ , Bingcong Xing _{1,

2} , Lijun Liang _{1,

2} , Bingxue Zhang _{1,

2} , Zongsuo Liang _{1,

2,

3}

Affiliation

S. miltiorrhiza is a well-known Chinese herb for the clinical treatment of cardiovascular and cerebrovascular diseases. Tanshinones and phenolic acids are the major secondary metabolites and significant pharmacological constituents of this plant. Kelch repeat F-box (KFB) proteins play important roles in plant secondary metabolism, but their regulation mechanism in S. miltiorrhiza has not been characterized. In this study, we systematically characterized the S. miltiorrhiza KFB gene family. In total, 31 SmKFB genes were isolated from S. miltiorrhiza. Phylogenetic analysis of those SmKFBs indicated that 31 SmKFBs can be divided into four groups. Thereinto, five SmKFBs (SmKFB1, 2, 3, 5, and 28) shared high homology with other plant KFBs which have been described to be regulators of secondary metabolism. The expression profile of SmKFBs under methyl jasmonate (MeJA) treatment deciphered that six SmKFBs (SmKFB1, 2, 5, 6, 11, and 15) were significantly downregulated, and two SmKFBs (SmKFB22 and 31) were significantly upregulated. Tissue-specific expression analysis found that four SmKFBs (SmKFB4, 11, 16, and 17) were expressed preferentially in aerial tissues, while two SmKFBs (SmKFB5, 25) were predominantly expressed in roots. Through a systematic analysis, we speculated that SmKFB1, 2, and 5 are potentially involved in phenolic acids biosynthesis.

中文翻译：

丹参Kelch重复F-box（KFB）蛋白基因家族的系统分析及酚酸调控成员的鉴定

丹参是临床治疗心脑血管疾病的著名中草药。丹参酮和酚酸是这种植物的主要次生代谢产物和重要的药理成分。Kelch 重复 F-box (KFB) 蛋白在植物次生代谢中发挥重要作用，但其在丹参中的调控机制尚未明确。在这项研究中，我们系统地表征了丹参 KFB 基因家族。总共从丹参中分离了 31 个 SmKFB 基因。这些SmKFBs的系统发育分析表明31个SmKFBs可分为四组。其中，5 个 SmKFB（SmKFB1、2、3、5 和 28）与其他植物 KFB 具有高度同源性，这些 KFB 已被描述为次生代谢的调节剂。茉莉酸甲酯 (MeJA) 处理下 SmKFB 的表达谱破译了六个 SmKFB（SmKFB1、2、5、6、11 和 15）显着下调，两个 SmKFB（SmKFB22 和 31）显着上调。组织特异性表达分析发现，四个 SmKFBs（SmKFB4、11、16 和 17）优先在地上组织中表达，而两个 SmKFBs（SmKFB5、25）主要在根中表达。通过系统分析，我们推测 SmKFB1、2 和 5 可能参与酚酸生物合成。和 17) 优先在地上组织中表达，而两个 SmKFBs (SmKFB5, 25) 主要在根中表达。通过系统分析，我们推测 SmKFB1、2 和 5 可能参与酚酸生物合成。和 17) 优先在地上组织中表达，而两个 SmKFBs (SmKFB5, 25) 主要在根中表达。通过系统分析，我们推测 SmKFB1、2 和 5 可能参与酚酸生物合成。

更新日期：2020-05-16

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