The objective of this study was in-depth identification of carotenoids and polyphenolic compounds in leaves and fruits of Elaeagnus multiflora Thunb. An additional aim was to assay their antioxidant and in vitro biological activities (the ability to inhibit pancreatic lipase, α-amylase, and α-glucosidase activity) of two cultivars: 'Sweet Scarlet' and 'Jahidka'. Study results showed the presence of 70 bioactive compounds, including 20 isoprenoids and 50 polyphenols. The profile of identified bioactive compounds had not been examined in this respect until now. The total carotenoid, chlorophyll, and polyphenol levels and antioxidant activity of the foliar samples were virtually identical in both cultivars and clearly higher relative to those in the fruits. On the other hand, the ability to inhibit pancreatic lipase, α-amylase, and α-glucosidase activity of the fruits was clearly higher as compared to the leaves. The highest amount of phenolic acids, flavonols, and polymeric procyanidins was in the 'Sweet Scarlet' for fruit and leaves, while the highest amount of chlorophylls and carotenoids was in the 'Jahidka'. The inhibition of α-amylase, α-glucosidase, and pancreatic lipase activities appeared to be better correlated with the carotenoid content, which warrants further studies of the possible anti-diabetic and anti-obesity actions of the major carotenoids found in the fruits (lycopene, phytoene, and lutein). In addition, strong correlation between antioxidant activity and phenols of E. multiflora Thunb. components can be effective in removing reactive oxygen species. The results of our study show that both the fruits and leaves of E. multiflora Thunb. can be important for health promotion through the diet and for innovating in the industry of functional food and (nutri)cosmetics.

中文翻译：

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多枝胡枝子果实和叶片的体外生物活性。以及它们的类异戊二烯和多酚谱。

这项研究的目的是深入鉴定何首乌叶和果实中的类胡萝卜素和多酚类化合物。另一个目的是测定两个品种的甜味和猩红蛋白的抗氧化和体外生物学活性（抑制胰脂肪酶，α-淀粉酶和α-葡萄糖苷酶的能力）。研究结果表明存在70种生物活性化合物，包括20种类异戊二烯和50种多酚。到目前为止，在这方面尚未检查鉴定出的生物活性化合物的概况。叶面样品的总类胡萝卜素，叶绿素和多酚水平以及抗氧化活性在两个品种中实际上是相同的，并且相对于果实中的明显更高。另一方面，抑制胰腺脂肪酶，α-淀粉酶，果实的α-葡萄糖苷酶活性明显高于叶片。水果和树叶的“甜红色”中酚酸，黄酮醇和聚合原花青素的含量最高，而“贾希德卡”中的叶绿素和类胡萝卜素含量最高。抑制α-淀粉酶，α-葡萄糖苷酶和胰腺脂肪酶的活性似乎与类胡萝卜素含量更好地相关，这值得进一步研究水果中发现的主要类胡萝卜素（番茄红素）可能的抗糖尿病和抗肥胖作用。 ，八氢番茄红素和叶黄素）。此外，抗氧化活性与何首乌酚之间的关系也很密切。组分可以有效去除活性氧。我们的研究结果表明，E。multiflora Thunb的果实和叶片均如此。