Synthesis of nanoparticles using the plants has several advantages over other methods due to the environmentally friendly nature of plants. Besides being environmentally friendly, the synthesis of nanoparticles using plants or parts of the plants is also cost effective. The present study focuses on the biosynthesis of zinc oxide nanoparticles (ZnO NPs) using the seed extract of Butea monsoperma and their effect on to the quorum-mediated virulence factors of multidrug-resistant clinical isolates of Pseudomonas aeruginosa at sub minimum inhibitory concentration (MIC). The synthesized ZnO NPs were characterized by different techniques, such as Fourier transform infra-red spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and transmission electron microscopy (TEM). The average size of the nanoparticles was 25 nm as analyzed by TEM. ZnO NPs at sub MIC decreased the production of virulence factors such as pyocyanin, protease and hemolysin for P. aeruginosa (p ≤ 0.05). The interaction of NPs with the P. aeruginosa cells on increasing concentration of NPs at sub MIC levels showed greater accumulation of nanoparticles inside the cells as analyzed by TEM.

中文翻译：

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生物合成的ZnO纳米颗粒对耐多药铜绿假单胞菌的影响。

由于植物的环境友好性质，使用植物合成纳米颗粒比其他方法具有多个优点。除了对环境友好之外，使用植物或植物的一部分来合成纳米颗粒也是成本有效的。本研究着重于使用mon子的种子提取物生物合成氧化锌纳米颗粒（ZnO NPs）及其对铜绿假单胞菌多药耐药临床分离株在低于最小抑制浓度（MIC）时对群体介导的毒力因子的影响。 。合成的ZnO NPs采用不同的技术进行表征，例如傅立叶变换红外光谱（FTIR），扫描电子显微镜（SEM），能量色散X射线（EDX）和透射电子显微镜（TEM）。通过TEM分析，纳米颗粒的平均尺寸为25nm。亚MIC处的ZnO NPs减少了铜绿假单胞菌的毒力因子（例如，花青素，蛋白酶和溶血素）的产生（p≤0.05）。通过TEM分析，NPs与铜绿假单胞菌相互作用增加了亚MIC水平下NPs浓度的增加。