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Molecular Typing, Characterization of Antimicrobial Resistance, Virulence Profiling and Analysis of Whole-Genome Sequence of Clinical Klebsiella pneumoniae Isolates.
Antibiotics ( IF 4.8 ) Pub Date : 2020-05-17 , DOI: 10.3390/antibiotics9050261
Andrey Shelenkov 1 , Yulia Mikhaylova 1 , Yuri Yanushevich 1 , Andrei Samoilov 1 , Lyudmila Petrova 2 , Valeria Fomina 2 , Vitaly Gusarov 2 , Mikhail Zamyatin 2 , Dmitriy Shagin 1, 3 , Vasiliy Akimkin 1
Affiliation  

Klebsiella pneumoniae is one of the most important pathogens concerned with multidrug resistance in healthcare-associated infections. The treating of infections caused by this bacterium is complicated due to the emergence and rapid spreading of carbapenem-resistant strains, which are associated with high mortality rates. Recently, several hypervirulent and carbapenemase-producing isolates were reported that make the situation even more complicated. In order to better understand the resistance and virulence mechanisms, and, in turn, to develop effective treatment strategies for the infections caused by multidrug-resistant K. pneumoniae, more comprehensive genomic and phenotypic data are required. Here, we present the first detailed molecular epidemiology report based on second and third generation (long-read) sequencing for the clinical isolates of K. pneumoniae in the Russian Federation. The data include three schemes of molecular typing, phenotypic and genotypic antibiotic resistance determination, as well as the virulence and plasmid profiling for 36 K. pneumoniae isolates. We have revealed 2 new multilocus sequence typing (MLST)-based sequence types, 32 multidrug-resistant (MDR) isolates and 5 colistin-resistant isolates in our samples. Three MDR isolates belonged to a very rare ST377 type. The whole genome sequences and additional data obtained will greatly facilitate further investigations in the field of antimicrobial resistance studies.

中文翻译:

临床肺炎克雷伯菌分离株的分子分型,抗药性鉴定,毒力谱分析和全基因组序列分析。

肺炎克雷伯菌是与医疗保健相关感染中的多药耐药性最重要的病原体之一。由这种细菌引起的感染的治疗由于对碳青霉烯抗性菌株的出现和迅速传播而变得复杂,这与高死亡率相关。最近,据报道有几种高毒力和碳青霉烯酶生产菌株,使情况更加复杂。为了更好地了解耐药性和毒力机制,并进而开发出针对由多药耐药的肺炎克雷伯菌引起的感染的有效治疗策略,需要更全面的基因组和表型数据。这里,我们提供了基于第二代和第三代(长读)测序的俄罗斯联邦肺炎克雷伯菌临床分离株的第一份详细的分子流行病学报告。数据包括分子分型,表型和基因型抗生素抗性测定的三种方案,以及36株肺炎克雷伯菌分离株的毒力和质粒图谱分析。我们在我们的样本中揭示了2种基于多基因座序列类型(MLST)的新序列类型,32种耐多药(MDR)分离株和5种耐大肠粘菌素的分离株。三个MDR分离株属于非常罕见的ST377类型。完整的基因组序列和获得的其他数据将极大地促进抗菌素耐药性研究领域的进一步研究。表型和基因型抗生素耐药性的测定,以及36株肺炎克雷伯菌分离株的毒力和质粒谱分析。我们在我们的样本中揭示了2种基于多基因座序列类型(MLST)的新序列类型,32种耐多药(MDR)分离株和5种耐粘菌素的分离株。三个MDR分离株属于非常罕见的ST377类型。完整的基因组序列和获得的其他数据将极大地促进抗菌素耐药性研究领域的进一步研究。表型和基因型抗生素耐药性的测定,以及36株肺炎克雷伯菌分离株的毒力和质粒谱分析。我们在我们的样本中揭示了2种基于多基因座序列类型(MLST)的新序列类型,32种耐多药(MDR)分离株和5种耐大肠粘菌素的分离株。三个MDR分离株属于非常罕见的ST377类型。完整的基因组序列和获得的其他数据将极大地促进抗菌素耐药性研究领域的进一步研究。三个MDR分离株属于非常罕见的ST377类型。完整的基因组序列和获得的其他数据将极大地促进抗菌素耐药性研究领域的进一步研究。三个MDR分离株属于非常罕见的ST377类型。完整的基因组序列和获得的其他数据将极大地促进抗菌素耐药性研究领域的进一步研究。
更新日期:2020-05-17
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