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A simple method for gene expression in endo- and ectodermal cells in mouse embryos before neural tube closure
bioRxiv - Developmental Biology Pub Date : 2023-03-04 , DOI: 10.1101/2020.05.14.086330
Naohiro Kuwayama , Yusuke Kishi , Yurie Maeda , Yurie Nishiumi , Yutaka Suzuki , Haruhiko Koseki , Yusuke Hirabayashi , Yukiko Gotoh

The lack of a widely accessible method for expressing genes of interest in wild-type embryos is the fundamental obstacle in understanding genetic regulation during embryonic development. In particular, only a few methods are available for introducing gene expression vectors into cells prior to neural tube closure, a period for the drastic development of many tissues. In this study, we present a simple technique for transferring vectors into the endo- and ectodermal cells of mouse embryos at E7.0 or E8.0 via in utero injection, without any specialized equipment. Using this technique, introduction of retroviruses can facilitate the labeling of cells in various tissues, including the brain, spinal cord, epidermis, and digestive and respiratory organs. As such, this technique can aid in analyzing the roles of genes of interest during endo- and ectodermal development prior to neural tube closure.

中文翻译:

神经管闭合前小鼠胚胎内胚层和外胚层细胞基因表达的简单方法

缺乏一种可广泛使用的表达野生型胚胎感兴趣基因的方法是理解胚胎发育过程中遗传调控的根本障碍。特别是,只有少数方法可用于在神经管闭合之前将基因表达载体引入细胞,这是许多组织急剧发育的时期。在这项研究中,我们提出了一种简单的技术,可通过子宫内注射将载体转移到 E7.0 或 E8.0 小鼠胚胎的内胚层和外胚层细胞中,无需任何专门设备。使用这种技术,逆转录病毒的引入可以促进各种组织中细胞的标记,包括大脑、脊髓、表皮以及消化和呼吸器官。像这样,
更新日期:2023-03-06
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