The aim of this experiment was to study the effects of dietary addition of high‐dose Moringa extract (10 g/kg Moringa extract) on laying performance, haematological parameters, organ index and organ histopathology of Hailan brown laying hens in order to determine the safety limit of Moringa extract in laying hens diet. 270 Hailan brown commercial laying hens with similar body weight were randomly divided into three treatments, 6 replicates per treatment and 15 chickens per replicate. Corn–soybean meal‐based diet was used in the experiment, and 0, 0.75 and 10 g/kg extracts of Moringa oleifera were added to the basic diet in the experimental group. The experiment lasted 24 weeks. The results showed that the treatment group given 10 g/kg of dietary Moringa extract had no significant difference in egg‐laying performance from the control group (p > .05). Adding 0.75 g/kg Moringa extract had no significant effect on the production performance during the early stage of the experiment (p > .05), but during the later stage, F/E was significantly lower than in the control group (p < .05), and no significantly difference compared with 10 g/kg treatment group (p > .05). The addition of Moringa oleifera extract had no significant effect on serum biochemical indices of laying hens except ALB and UREA (p > .05). The addition of Moringa oleifera extract had no significant effect on blood routine indexes of laying hens except for PLT, MON and HCT (p > .05). The digestive and immune organ indexes of laying hens at the later stage of the experiment (24 weeks) were not significantly affected by the addition of Moringa extract (p > .05) except ceca. There were no obvious abnormalities in histological structure. On the whole, we find it is safe to add Moringa oleifera extract to the diet of laying hens at a dosage of less than 10 g/kg.

中文翻译：

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辣木提取物对海兰棕蛋鸡的耐受性评价。

本实验的目的是研究日粮添加大剂量辣木提取物（10 g / kg辣木提取物）对海兰褐蛋鸡产蛋性能，血液学参数，器官指标和器官组织病理学的影响，以确定安全性蛋鸡饮食中辣木提取物的限量。将270头体重相似的海兰棕色商品蛋鸡随机分为3种处理，每处理6个重复，每重复15只鸡。在实验中使用了以玉米-豆粕为基础的饮食，在实验组的基础饮食中添加了辣木提取物0、0.75和10 g / kg 。实验持续了24周。结果表明，治疗组给予10 g / kg的饮食辣木提取物与对照组的产卵性能没有显着差异（p  > .05）。在实验初期，添加0.75 g / kg辣木提取物对生产性能没有显着影响（p  > .05），但在后期，F / E显着低于对照组（p  <。 05），与10 g / kg治疗组相比无明显差异（p  > .05）。除了ALB和尿素外，添加辣木提取物对蛋鸡的血清生化指标没有显着影响（p  > .05）。辣木的添加除PLT，MON和HCT外，提取物对蛋鸡的血液常规指标没有显着影响（p  > .05）。在实验的后期（24周），蛋鸡的采食和免疫器官指数不受 盲肠除辣木提取物的影响（p > .05）。组织结构无明显异常。总体而言，我们发现以低于10 g / kg的剂量将辣木提取物添加到蛋鸡的饮食中是安全的。