Gonadotropin‐releasing hormone (GnRH) is a key molecule in the initiation of the hypothalamic–pituitary–gonadal axis. Thus, knowledge about GnRH may contribute to the effectiveness of species reproduction. Using a Neotropical tetra Astyanax altiparanae as a fish model species, the GnRH forms were characterized at the molecular level and the role of injected GnRHs in vivo was evaluated. The full‐length complementary DNA (cDNA) sequences of preproGnRH2 (612 bp) and preproGnRH3 (407 bp) of A. altiparanae were obtained, and the GnRH1 form was not detected. The cDNA sequences of preproGnRH2 and preproGnRH3 were found to be conserved, but a change in the amino acid at position 8 of the GnRH3 decapeptide of A. altiparanae was observed. All the injected GnRHs stimulated lh β messenger RNA (mRNA) expression but not fsh β mRNA expression, and only GnRH2 was able to increase maturation‐inducing steroid (MIS) levels and possibly stimulate oocyte release. Furthermore, only GnRH2 was able to start the entire reproductive hormonal cascade and induce spawning.

中文翻译：

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鼠疫（Characiformes）中不同preproGnRHs的分子特征：GnRH对雌性生殖的影响。

促性腺激素释放激素（GnRH）是下丘脑-垂体-性腺轴启动的关键分子。因此，有关GnRH的知识可能有助于物种繁殖的有效性。使用新热带四叶连翘作为鱼类模型物种，在分子水平上表征了GnRH的形式，并评估了体内注射的GnRH的作用。preproGnRH2（612碱基对）和preproGnRH3（407 bp）的的全长互补DNA（cDNA）序列A. altiparanae获得，并且没有检测到GNRH1形式。发现preproGnRH2和preproGnRH3的cDNA序列是保守的，但是拟南芥GnRH3十肽第8位的氨基酸发生了变化。被观测到。所有注射GnRHs刺激LH β信使RNA（mRNA）的表达，但不FSH βmRNA的表达，只有GnRH2能够增加细胞成熟诱导激素（MIS）水平，并可能刺激卵母细胞释放。此外，只有GnRH2能够启动整个生殖激素级联并诱导产卵。