3D bioprinting is a promising new tissue restoration technique that enables the precise deposition of cells and growth factors in order to more closely mimic the structure and function of native organs. In this study, we report the development of a new bioink using oligo(poly[ethylene glycol] fumarate) (OPF), a photo‐crosslinkable, and biodegradable polymer, for 3D bioprinting. In addition to OPF, a small portion of gelatin was also incorporated into the bioink to make it bio‐printable. After immersion in the cell medium, gelatin was eluted away to create a bioprinted scaffold of pure OPF. Excellent cell viability, spreading, and long‐term proliferation of encapsulated cells was observed using both bone and nerve cells as examples. These results demonstrate that OPF bioink has great potential in future 3D bioprinting applications that aim to replicate complex, layered tissues, and/or organs.

中文翻译：

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用于骨骼和神经组织工程的寡聚（聚[乙二醇]富马酸酯）的 3D 生物打印。

3D 生物打印是一种很有前途的新型组织修复技术，它能够精确地沉积细胞和生长因子，从而更接近地模拟天然器官的结构和功能。在这项研究中，我们报告了一种使用寡聚（聚[乙二醇]富马酸酯）（OPF）（一种可光交联和可生物降解的聚合物）用于 3D 生物打印的新型生物墨水的开发。除了 OPF，一小部分明胶也被掺入到生物墨水中，使其可进行生物打印。浸入细胞培养基后，将明胶洗脱下来，形成纯 OPF 的生物打印支架。以骨细胞和神经细胞为例，观察到包裹细胞具有出色的细胞活力、扩散和长期增殖。