This 27‐color flow cytometry panel was developed in order to assess immunological changes over the course of an immunization and challenge regimen in two experimental malaria vaccine trials. The aim of the study was to find correlates of vaccine‐induced protection. Several studies have indicated that protection against malaria appears to involve immune responses at various immunological sites, with liver‐resident responses playing an essential role. As it is not feasible to monitor the immune responses within the liver in humans, this panel is developed with the aim to thoroughly characterize the immune responses over time in blood in addition to detecting changes that might reflect what happens in other immunological sites like the liver. The focus of this panel is to detect several innate lymphoid cell populations, including NK cells and their activation status. Moreover, unconventional T cells like mucosal associated invariant T cells and γδ T cells are assessed in the panel. © 2020 International Society for Advancement of Cytometry

中文翻译：

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OMIP-064：用于检测和表征人类 NK 细胞和其他先天淋巴细胞亚群、MAIT 细胞和 γδ T 细胞的 27 色流式细胞术面板。

开发这种 27 色流式细胞术面板是为了在两个实验性疟疾疫苗试验中评估免疫和攻击方案过程中的免疫学变化。该研究的目的是寻找疫苗诱导保护的相关性。几项研究表明，对疟疾的保护似乎涉及不同免疫位点的免疫反应，肝脏驻留反应起着至关重要的作用。由于监测人体肝脏内的免疫反应是不可行的，因此开发该面板的目的是除了检测可能反映肝脏等其他免疫部位发生的变化之外，还可以彻底表征血液中随时间推移的免疫反应. 该小组的重点是检测几种先天淋巴细胞群，包括 NK 细胞及其激活状态。此外，非常规 T 细胞，如粘膜相关的不变 T 细胞和 γδ T 细胞在面板中进行评估。© 2020 国际细胞计量学促进会