Here we report on the structure and mechanical properties of native bovine jugular veins (BJVs). The veins were pretreated with co-solvents in supercritical (sc) CO₂ and cross-linked by glutaraldehyde veins coated with chitosan from solutions in carbonic acid). The novel hybrid treatment method was developed using rapid processing in SDS (1 h) and treatment in scCO₂ adding the non-ionic detergent (Tween-80) (1 h). The hybrid method allows to decellularize the tissue completely, preserving the structure of the extracellular matrix, and at the same time to significantly increase the elasticity of vein tissue, bringing it closer to the human pulmonary artery in its properties (E∼8 MPa). Protective chitosan-based coatings, deposited onto the tissue (∼0.8 wt% for chitosan, 0.45% for chitosan/Ag nanoparticles (AgNPs)) from the water/subcritical CO₂ system (3 h), mask free aldehyde groups, smooth the surface of veins and maintain the elastic properties of the tissue.

在这里，我们报告天然牛颈静脉（BJVs）的结构和力学性能。这些静脉在超临界（sc）CO ₂中用助溶剂进行了预处理，并由涂有脱乙酰壳多糖的戊二醛静脉交联而成，这些戊二醛静脉来自碳酸溶液。通过在SDS中快速处理（1 h）和在scCO _2中进行处理，开发了新的混合处理方法加入非离子型去污剂（Tween-80）（1 h）。混合法可以使组织完全脱细胞，保留细胞外基质的结构，同时显着增加静脉组织的弹性，使其特性更接近人肺动脉（E〜8 MPa）。从水/亚临界CO ₂系统（3 h）沉积到组织上的保护性壳聚糖基涂料（壳聚糖约0.8 wt％，壳聚糖/ Ag纳米颗粒（AgNPs）约0.45％），掩盖游离醛基，使表面光滑并保持组织的弹性。