Abstract Fructooligosaccharides (FOS) have gained significant attention for their prebiotic properties. Given that sugarcane syrup (SS) is sucrose-rich but with other nutritional benefits, its direct transformation into FOS may add value to this product. Therefore, the aims of this study were to develop FOS conversion from SS and to define the kinetic behaviour of FOS synthesized in a 1-L specialized pH-stat bioreactor (SPSB). The SS was composed of sucrose (58.93%) with considerable antioxidant capacities and Ƴ-aminobutyric acid. The developed SPSB process consisted of three stages: evaporation of sugarcane juice into syrup (68–75 °Brix) (stage 1), optimization of the Viscozyme L and SS mixture at different reaction temperatures (47–55 °C) (stage 2), and upscaling of the optimized reaction system under defined conditions in a 1 L-SPSB system (stage 3). In the 1 L-SPSB system, the enzymatic reaction yielded 32.22% of FOS from SS after a 6 h reaction, which is comparable with a pure system containing an equivalent concentration of 10% of sucrose as initial substrate with 39.55% yield. This result demonstrated the efficient conversion of SS into FOS, supporting the utilization of sugarcane juice for its health benefits.

中文翻译：

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使用专门的酶促 pH-stat 生物反应器从甘蔗糖浆中转化新型低聚果糖

摘要 低聚果糖 (FOS) 因其益生元特性而备受关注。鉴于甘蔗糖浆 (SS) 富含蔗糖但具有其他营养益处，将其直接转化为 FOS 可能会增加该产品的价值。因此，本研究的目的是开发 SS 的 FOS 转化，并定义在 1-L 专用 pH 稳定生物反应器 (SPSB) 中合成的 FOS 的动力学行为。SS 由具有相当抗氧化能力的蔗糖 (58.93%) 和 Ƴ-氨基丁酸组成。开发的 SPSB 工艺包括三个阶段：将甘蔗汁蒸发成糖浆（68-75°Brix）（阶段 1），在不同反应温度（47-55°C）下优化 Viscozyme L 和 SS 混合物（阶段 2） , 并在 1 L-SPSB 系统（第 3 阶段）中的定义条件下升级优化的反应系统。在 1 L-SPSB 系统中，6 小时反应后，酶促反应从 SS 中产生了 32.22% 的 FOS，这与含有等量浓度 10% 蔗糖作为初始底物的纯系统相当，产率为 39.55%。这一结果表明 SS 有效转化为 FOS，支持利用甘蔗汁对健康有益。