Abstract In the present investigation, the role of dietary intervention in male Wistar rats (n = 8, 3 groups) was studied to observe absolute sirtuin 1 (SIRT1) levels (expressed as ng mg−1 total protein) in serum, stomach, liver, and kidney. Dietary buckwheat at 30% (w/w) level of incorporation in the standard diet (Buckwheat Enriched Diet, BED) improved SIRT1 with values 0.933 ± 0.05, 210 ± 7, 63.26 ± 4, and 69.89 ± 3 in serum, stomach, liver, and kidney respectively when compared to the respective control values of 0.536 ± 0.03, 156 ± 23.3, 31.07 ± 2 and 47.11 ± 4. Moreover, BED though isocaloric to CR diet, led to weight gain (g) by 63.11 ± 3.8, ca. 10%, and 40% higher than control (56.27 ± 5.6) and CR (45.05 ± 4.1) diet groups. A marked rise in Feed Efficiency Ratio (FER) by ca. 37% while a 30% decrease in Feed Conversion Ratio (FCR) was observed for the BED group which supports unexpected weight gain in rats post-dietary intervention. The results justify the superior nutritional profile of buckwheat laden with essential nutrients, essential proteins, and bioactives. In contrast, Calorie Restriction (CR) resulted in a decline of the total protein content in circulation by 19%, while reduction of total protein in stomach, liver, and kidney was estimated to be 95%, 35.2%, and 27% respectively though SIRT1 values were comparatively the highest in all the samples studied. A 30-fold enhancement of SIRT1 in stomach post CR is presumed to counter enhanced stress in gastric tissues. Therefore, mild to moderate expression of SIRT1 may confer beneficial effects such as delayed aging and stress resistance but exceedingly high SIRT1 may evoke increased oxidative stress.

中文翻译：

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膳食荞麦在没有卡路里限制的情况下增强sirtuin1

摘要 在本研究中，研究了饮食干预对雄性 Wistar 大鼠（n = 8, 3 组）的作用，以观察血清、胃、肝脏中的绝对 Sirtuin 1 (SIRT1) 水平（表示为 ng mg-1 总蛋白）。 ，和肾脏。在标准饮食（Buckwheat Enriched Diet，BED）中掺入 30% (w/w) 水平的饮食荞麦改善了 SIRT1，血清、胃、肝脏中的值分别为 0.933 ± 0.05、210 ± 7、63.26 ± 4 和 69.89 ± 3和肾脏分别与 0.536 ± 0.03、156 ± 23.3、31.07 ± 2 和 47.11 ± 4 的各自对照值相比。此外，BED 虽然与 CR 饮食等热量，但导致体重增加 (g) 63.11 ± 3.8，约. 比对照 (56.27 ± 5.6) 和 CR (45.05 ± 4.1) 饮食组高 10% 和 40%。饲料效率比 (FER) 显着上升约。37% 而观察到 BED 组的饲料转化率 (FCR) 下降了 30%，这支持了饮食干预后大鼠意外体重增加。结果证明荞麦富含必需营养素、必需蛋白质和生物活性物质的卓越营养成分是合理的。相比之下，卡路里限制 (CR) 导致循环中总蛋白质含量下降 19%，而胃、肝脏和肾脏中的总蛋白质含量估计分别减少 95%、35.2% 和 27%，尽管在所有研究的样品中，SIRT1 值相对最高。据推测，CR 后胃中 SIRT1 的 30 倍增强被认为可以对抗胃组织中增强的压力。所以，