Abstract Berberis mikuna is a spontaneous shrub grown in the Tucuman-Bolivian subtropical jungle area and its fruits were highly prized as food by diaguitas and calchaquies who were local populations. Fruits are rich in phenolic compounds which would be responsible for the beneficial properties for health. Pollen preservation in gene banks should be implemented as standard procedures since pollen is a useful source of diverse alleles within a gene pool. Therefore, the aim of this work was to study the histological and ultrastructural changes of pollen grains after 45 days of conservation at different temperatures. Berberis mikuna pollen grain germination and viability decreased significantly after 45 days of its collection. Germination and viability decreased with increasing storage temperature, although the viability in T45 Ref and T45 Fr was similar. Ultrastructure studies revealed differences in the fibrillar structure of the intine, as well as the decrease in starch reserve in pollen grains stored at 5 °C andat room temperature.

中文翻译：

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小檗花粉粒的组织学变化与活力和发芽性的关系

摘要 小檗小檗是生长在土库曼-玻利维亚亚热带丛林地区的一种自发灌木，其果实被当地居民diaguitas和calchaquies高度评价为食物。水果富含酚类化合物，对健康有益。基因库中的花粉保存应作为标准程序实施，因为花粉是基因库中各种等位基因的有用来源。因此，这项工作的目的是研究花粉粒在不同温度下保存 45 天后的组织学和超微结构变化。在收集 45 天后，小檗花粉粒的萌发率和活力显着下降。发芽率和生存力随储存温度升高而降低，尽管 T45 Ref 和 T45 Fr 的生存能力相似。超微结构研究揭示了内脏的原纤维结构的差异，以及在 5°C 和室温下储存的花粉粒中淀粉储备的减少。