Persicaria maculosa (Polygonaceae) has been used as edible and as medicinal plant since ancient times. As a result of multistep chromatographic purifications, chalcones [2'-hydroxy-3',4',6'-trimethoxychalcone (1), pashanone (2), pinostrobin chalcone (3)], flavanones [6-hydroxy-5,7-dimethoxyflavanone (4), pinostrobin (5), onysilin (6), 5-hydroxy-7,8-dimethoxyflavanone (7)], flavonol [3-O-methylgalangin (8)], stilbene [persilben (9)], diarylheptanoids [1,7-diphenylhept-4-en-3-one (10), dihydroyashabushiketol (12), yashabushidiol B (13)] and 3-oxo-α-ionol-glucoside (11) were isolated from P. maculosa. The present paper reports for the first time the occurrence of diarylheptanoid-type constituents in the family Polygonaceae. Cytotoxicity of 1-5, 7 and 9-11 on 4 T1 mouse triple negative breast cancer cells was assayed by MTT test. None of the tested compounds reduced the cell viability to less than 80% of the control. On non-tumorigenic D3 human brain endothelial cells the decrease of cell viability was observed in case of 1 and 2. Further impedance measurements on 4 T1 and D3 cells a concentration-dependent decrease in the cell index of both cell types was demonstrated for 1, while 2 proved to be toxic only on endothelial cells.

中文翻译：

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黄花Persicaria maculosa Gray的类黄酮，二苯乙烯和二芳基庚烷成分以及分离出的化合物的细胞毒活性。

Persicaria maculosa（Polygonaceae）自古以来就被用作食用和药用植物。经过多步色谱纯化后，查耳酮[2'-羟基-3'，4'，6'-三甲氧基查尔酮（1），帕沙酮（2），松油菌素查尔酮（3）]，黄烷酮[6-羟基-5,7 -二甲氧基黄酮（4），油灰黄tro（5），Onysilin（6），5-羟基-7,8-二甲氧基黄酮（7），黄酮醇[3-O-甲基高良姜精（8）]，[Persilben（9）]从青斑假单胞菌中分离出二芳基庚烷类化合物[1,7-二苯基庚-4-en-3-one（10），二氢Yayabushiketol（12），Yashabushidiol B（13）]和3-氧代-α-紫罗兰糖苷（11）。本论文首次报道了gon科中二芳基庚烷型成分的出现。通过MTT测试测定1-5、7和9-11对4只T1小鼠三阴性乳腺癌细胞的细胞毒性。所测试的化合物均未将细胞活力降低至对照的80％以下。在非致瘤性D3人脑内皮细胞中，在1和2的情况下观察到了细胞活力的降低。进一步测量了4个T1和D3细胞的阻抗，发现两种细胞类型的细胞指数均依赖浓度降低，即1 2个仅对内皮细胞有毒。