Based on a method combining the LC-MS/MS molecular networking strategy with the conventional means of phytochemical research, the chemical constituents and the availability of Paris tengchongensis, a new species found in 2017 from Yunnan Province, were investigated for the first time. The molecular networking showed that this species contained the characteristic steroidal glycosides of the genus Paris by comparison of those of Paris polyphylla var. yunnanensis. Furthermore, the detailed investigation on the 80% EtOH extract of its rhizomes resulted to the isolation of twenty steroidal glycosides including three new spirostane-type saponins, named paristengosides A-C (1–3). Their structures were confirmed by spectroscopic analyses (HRMS and NMR) and chemical methods. The new isolates were evaluated for their cytotoxicities against two human cancer cell lines (HEL and MDA-MB-231), anti-inflammatory effects on a lipopolysaccharide (LPS)-stimulated NO production model in RAW264.7 macrophages, anti-AChE, and antimicrobial activities. The results from the molecular networking and the investigation on the chemical constituents suggested that P. tengchongensis can be used as a potential resource of Rhizoma Paridis.

基于将LC-MS / MS分子联网策略与常规植物化学研究手段相结合的方法，首次调查了云南省2017年发现的新物种巴黎腾冲树的化学成分和可用性。分子网络表明，与巴黎多叶植物的巴黎树相比，该物种包含巴黎属的特征性甾体糖苷。云南。此外，对其根茎中80％EtOH提取物的详细研究导致分离出20种甾体糖苷，其中包括三种新的螺环烷型皂苷，称为paristengosides AC（1-3）。通过光谱分析（HRMS和NMR）和化学方法确认了它们的结构。评估了新分离株对两种人类癌细胞系（HEL和MDA-MB-231）的细胞毒性，在RAW264.7巨噬细胞中对脂多糖（LPS）刺激的NO产生模型的抗炎作用，抗AChE和抗菌活性。分子网络和化学成分研究的结果表明，P。腾冲可以作为根茎的潜在资源。