This study aimed to assess the effect of supercritical carbon dioxide (SC-CO₂) on the enzymatic hydrolysis of sugarcane bagasse to produce fermentable sugars. A central composite design (CCD) was used to investigate the influence of temperature (40 to 60 °C), pressure (100 to 300 bar), and reaction time (60 to 180 min) on enzymatic hydrolysis using 2% (w v⁻¹) sugarcane bagasse and a commercial cellulase. After supercritical treatment, samples were kept at 50 °C for 24 h to complete the hydrolysis reaction. SC-CO₂ treatment at 200 bar and 50 °C for 120 min increased the release of fermentable sugars by 32.49% compared with the control (without SC-CO₂). The condition (50 °C, 200 bar, 120 min) that provided the highest concentration of reducing sugars (2.340 g L⁻¹) resulted in a hydrolysis yield of 32.4%. SC-CO₂ treatment is a sustainable strategy to enhance the enzymatic hydrolysis of lignocellulosic biomass and to intensify the release of fermentable sugars.

这项研究旨在评估超临界二氧化碳（SC-CO ₂）对甘蔗渣的酶解产生可发酵糖的影响。中央复合设计（CCD）用于研究温度（40至60°C），压力（100至300 bar）和反应时间（60至180分钟）对使用2％（wv ^-1）的酶水解的影响）甘蔗渣和商业纤维素酶。超临界处理后，将样品在50°C下放置24小时以完成水解反应。与对照组相比，SC-CO ₂在200 bar和50°C下处理120分钟可发酵糖的释放增加32.49％（无SC-CO ₂）。提供最高浓度的还原糖（2.340 g L ^-1）的条件（50°C，200 bar，120分钟）导致水解产率为32.4％。SC-CO ₂处理是增强木质纤维素生物质的酶促水解并增强可发酵糖释放的可持续策略。