Very few studies to date have focused on phosphorus (P) dynamics and microbial P acquisition during anaerobic soil disinfestation (ASD), an alternative to chemical soil fumigation for soil diseases. We evaluated the effects of ASD using diluted ethanol as well as the effects of drying treatments following ASD on available P concentrations, phosphomonoesterase activity, microbial biomass P, and microbial community compositions, including those harboring the alkaline phosphomonoesterase gene (phoD), in two arable Andosols. An increase in the Bray-2P concentration and a decrease in the ratio of phosphomonoesterase/β-D-glucosidase activities were observed during ASD in both soils, indicating an increase in P availability during ASD. A significant negative correlation between the diversity of profile of denaturing gradient gel electrophoresis for phoD-harboring community and alkaline phosphomonoesterase activity was found, suggesting that a less diverse bacterial group might produce alkaline phosphomonoesterase in soils with higher activity. ASD followed by drying and rewetting significantly increased the Bray-2P concentration and microbial biomass P in one Andosol. These results support the potential of ASD to increase P availability in addition to suppressing soil-borne diseases in arable Andosols.

中文翻译：

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使用稀释的乙醇进行厌氧土壤消毒增加可耕地安多溶胶中磷的有效性

迄今为止，很少有研究关注厌氧土壤消毒 (ASD) 过程中磷 (P) 的动态和微生物 P 的获取，这是土壤病害化学土壤熏蒸的替代方法。我们评估了使用稀释乙醇的 ASD 的影响以及 ASD 后干燥处理对可用 P 浓度、磷酸单酯酶活性、微生物生物量 P 和微生物群落组成的影响，包括那些携带碱性磷酸单酯酶基因 (phoD) 的两个耕地安多溶胶。在 ASD 期间观察到两种土壤中 Bray-2P 浓度的增加和磷酸单酯酶/β-D-葡萄糖苷酶活性比率的降低，表明 ASD 期间磷的有效性增加。发现phoD-harboring群落的变性梯度凝胶电泳图谱多样性与碱性磷酸单酯酶活性呈显着负相关，表明多样性较低的细菌群可能在活性较高的土壤中产生碱性磷酸单酯酶。ASD 然后干燥和再润湿显着增加了一种 Andosol 中的 Bray-2P 浓度和微生物生物量 P。这些结果支持 ASD 除了抑制可耕地 Andosols 中的土壤传播疾病外，还具有增加磷可用性的潜力。ASD 然后干燥和再润湿显着增加了一种 Andosol 中的 Bray-2P 浓度和微生物生物量 P。这些结果支持 ASD 除了抑制可耕地 Andosols 中的土壤传播疾病外，还具有增加磷可用性的潜力。ASD 然后干燥和再润湿显着增加了一种 Andosol 中的 Bray-2P 浓度和微生物生物量 P。这些结果支持 ASD 除了抑制可耕地 Andosols 中的土壤传播疾病外，还具有增加磷可用性的潜力。