Background

The greatest challenge of the day is the rising threat of neonatal sepsis as well as antimicrobial resistance. Accordingly, attention has been especially devoted to marine macroalgae metabolites, which are known to have prominent medicinal properties with major phytoconstituents. The object of this study is to evaluate the efficacy of three different macroalgal extracts (ME) as new ingredients classes exposed against multidrug-resistant (MDR) Klebsiella pneumoniae isolated from blood specimens of neonatal sepsis, as well as to evaluate the bio-compounds present in ME using gas chromatography–mass spectrometry (GC–MS).

Materials and Methods

Out of 370 neonates, only patients with positive blood culture 110 (29.7%) were enrolled. From clinical specimens, Klebsiella pneumoniae (n = 40) were recovered and were biochemically identified. Antimicrobial susceptibilities of the selected isolates to different antimicrobials were conducted using disk diffusion methods. Antibacterial activities of different AE, Ulva fasciata, Sargassum vulgare, Corallina officinalis, were investigated using agar well diffusion method. Furthermore, the constituents of the most promising ME were detected using GC–MS analysis.

Results

Susceptibility testing showed that all K. pneumoniae isolates were MDR with a great prevalence of resistance, especially to ampicillin and amoxicillin/clavulanic acid. Methanolic extract of S. vulgare exhibited the highest antibacterial activities. GC–MS data of the selected extract revealed eleven different secondary metabolites, which were mostly composed of organic acids.

Conclusion

The methanolic extract of the marine alga S. vulgare could be a potential candidate for the natural compounds as antibacterial therapy.

中文翻译：

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大型藻类提取物作为辅助治疗肺炎克雷伯菌引起的败血症的评价

背景

当今最大的挑战是新生儿败血症以及抗菌素耐药性的上升威胁。因此，人们特别关注海洋大型藻类代谢产物，已知这些代谢产物具有主要的植物成分，并且具有突出的药用特性。这项研究的目的是评估三种不同的大型藻类提取物（ME）作为对抗从新生儿败血症血液样本中分离出的耐多药（MDR）肺炎克雷伯菌的新成分类别的功效，并评估现有的生物化合物使用气相色谱-质谱法（GC-MS）测定。

材料和方法

在370名新生儿中，只有110名血液培养阳性的患者（29.7％）入选。从临床标本 中回收肺炎克雷伯菌（n = 40）并进行生化鉴定。使用圆盘扩散法进行所选分离株对不同抗菌素的抗菌敏感性。采用琼脂微孔扩散法研究了不同的AE，筋膜锦囊藻，小叶猴藻，Corallina officinalis的抗菌活性。此外，使用GC-MS分析检测了最有前途的ME的成分。

结果

药敏试验表明，所有肺炎克雷伯菌分离株均为耐多药的MDR，尤其是对氨苄西林和阿莫西林/克拉维酸的耐药性高。的甲醇提取物S.大麦表现出最高的抗菌活性。所选提取物的GC-MS数据显示了11种不同的次生代谢物，它们主要由有机酸组成。

结论

海藻小球藻的甲醇提取物可能是天然化合物作为抗菌疗法的潜在候选者。