Watermelon mosaic virus (WMV) is an important virus causing adverse effects on cucurbits throughout the world. In this study, we recorded WMV infection in the watermelon (Citrullus lanatus)-growing area of Alwar and Sikar in districts of Rajasthan, India. The RT-PCR-based detection was performed to confirm the presence of WMV, by using potyvirus-degenerated coat protein primers. Further, the complete genome sequences of two WMV isolates were compared with previously reported genome sequences. The complete genome of each isolate was 10,030 nt long, excluding the poly-A tails. Phylogeny relationships of the WMV isolates in the present study revealed the presence of uneven evolutionary pressure among the different WMV viral genomic segments. The analysis revealed that all the WMV isolates were divided into three clusters and the Indian WMV isolates cluster together with the French isolate. Recombination analysis of WMV exhibited significant recombination hotspots in the P1, NIa-Pro and Nib-CP regions. Our finding highlights the importance of genetic variability and recombination analysis to provide a better understanding of WMV molecular diversity.

中文翻译：

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西瓜花叶病毒分离株的鉴定，遗传多样性和重组分析。

西瓜花叶病毒（WMV）是一种重要的病毒，会对全世界的葫芦科产生不利影响。在这项研究中，我们记录了印度拉贾斯坦邦的Alwar和Sikar西瓜种植区（Citrullus lanatus）的WMV感染情况。通过使用马铃薯花叶病毒变性的外壳蛋白引物进行基于RT-PCR的检测，以确认WMV的存在。此外，将两个WMV分离株的完整基因组序列与先前报道的基因组序列进行了比较。每个分离株的完整基因组长度为10,030 nt，不包括poly-A尾巴。在本研究中，WMV分离株的系统发生关系揭示了不同WMV病毒基因组片段之间存在不均匀的进化压力。分析显示，所有WMV分离株均分为三个簇，印度WMV分离株与法国分离株一起。WMV的重组分析在P1，NIa-Pro和Nib-CP地区表现出明显的重组热点。我们的发现强调了遗传变异性和重组分析对更好地了解WMV分子多样性的重要性。