The glutathione (GSH) level in human serum is closely associated with several life-threatening diseases, and tracing the aberrant GSH level can monitor the subhealth conditions at an early stage for human health prognosis. Developing portable and direct read-out mini-devices is an inevitable trend for reliable point-of-care (POC) detection of GSH in real-time/on-site conditions. We herein report a portable smartphone-sensing platform, a ratiometric fluorescence sensor combined with the 3D-printed smartphone device for rapid, sensitive, quantitative, and on-the-spot determination of GSH in human serum. The powerful fluorescence “off–on” nanoprobe was constructed by mixing the blue carbon dots (CDs) and orange gold nanoclusters (AuNCs) with the assistance of copper ions. The quenched fluorescence can be quickly restored upon interacting with GSH, showing a distinct color variation from blue to purple to orange. Integrated with the paper strip printed by the probe ink, the smartphone platform installed with a Color Recognizer App could accomplish sensitive, reliable, and real-time/on-site detection of GSH in human serum, which shows great significance for early disease diagnosis. The constructed smartphone platform is expected to develop into portable home medical equipment to realize convenient and rapid preliminary monitoring and self-assessment of health.

中文翻译：

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便携式智能手机平台与基于纳米探针的荧光纸条集成：可视监测人体血清中的谷胱甘肽对健康的预后

人血清中的谷胱甘肽（GSH）水平与几种威胁生命的疾病密切相关，追踪异常的GSH水平可以在人类健康预后的早期阶段监测亚健康状况。开发便携式和直接读出的微型设备是在实时/现场条件下可靠地对GSH进行即时医疗（POC）检测的必然趋势。我们在此报告了一种便携式智能手机传感平台，一种比例荧光传感器与3D打印智能手机设备相结合，可快速，灵敏，定量和现场测定人血清中的GSH。通过将蓝碳点（CD）和橙金纳米团簇（AuNCs）与铜离子混合，可以构建强大的荧光“开-关”纳米探针。与GSH相互作用后，猝灭的荧光可以快速恢复，显示出从蓝色到紫色再到橙色的明显颜色变化。安装了Color Recognizer App的智能手机平台与探针墨水打印的纸条集成在一起，可以实现灵敏，可靠，实时/现场检测人血清中的GSH，这对早期疾病诊断具有重要意义。建成的智能手机平台有望发展成为便携式家用医疗设备，以实现便捷，快速的初步监测和健康自我评估。