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Antimicrobial Peptide GH12 Prevents Dental Caries by Regulating Dental Plaque Microbiota.
Applied and Environmental Microbiology ( IF 4.4 ) Pub Date : 2020-07-02 , DOI: 10.1128/aem.00527-20
Wentao Jiang 1, 2 , Yufei Wang 1, 2 , Junyuan Luo 1, 2 , Xiangshu Chen 1 , Yuhao Zeng 1, 2 , Xinwei Li 1, 2 , Zening Feng 1, 2 , Linglin Zhang 2, 3
Affiliation  

Due to the complex microecology and microenvironment of dental plaque, novel caries prevention strategies require modulating the microbial communities ecologically and reducing the cariogenic properties effectively. Antimicrobial peptide GH12 reduced the lactic acid production and exopolysaccharide (EPS) synthesis of a Streptococcus mutans biofilm and a three-species biofilm in vitro in previous studies. However, the anticaries effects and microecological effects of GH12 remained to be investigated in a complex biofilm model in vitro and an animal caries model in vivo. In the present study, GH12 at 64 mg/liter showed the most effective inhibition of lactic acid production, EPS synthesis, pH decline, and biofilm integrity of human dental plaque-derived multispecies biofilms in vitro, and GH12 at 64 mg/liter was therefore chosen for use in subsequent in vitro and in vivo assays. When treated with 64-mg/liter GH12, the dental plaque-derived multispecies biofilms sampled from healthy volunteers maintained its microbial diversity and showed a microbial community structure similar to that of the control group. In the rat caries model with a caries-promoting diet, 64-mg/liter GH12 regulated the microbiota of dental plaque, in which the abundance of caries-associated bacteria was decreased and the abundance of commensal bacteria was increased. In addition, 64-mg/liter GH12 significantly reduced the caries scores of sulcal and smooth surface caries in all locations. In conclusion, GH12 inhibited the cariogenic properties of dental plaque without perturbing the dental plaque microbiota of healthy individuals and GH12 regulated the dysbiotic microbial ecology and arrested caries development under cariogenic conditions.

中文翻译:

抗菌肽GH12通过调节牙菌斑微生物群来预防龋齿。

由于牙菌斑具有复杂的微生态学和微环境,因此,新型的防龋策略需要生态调节微生物群落并有效降低其致龋性。抗微生物肽GH12降低乳酸生产和胞外多糖(EPS)的一个合成变形链球菌生物膜和三物种生物膜在体外在以前的研究。然而,GH12的防龋作用和微生态作用仍有待在体外复杂的生物膜模型和体内动物龋齿模型中进行研究。在本研究中,GH12在64毫克/升显示出最有效的抑制乳酸生产,EPS合成,pH值下降,和人牙斑衍生的多物种生物膜的生物膜完整性体外,和GH12在64毫克/升是因此选择用于随后的体外体内分析。当用64 mg / L GH12处理时,从健康志愿者那里采集的牙菌斑来源的多种生物膜保持了其微生物多样性,并显示出与对照组相似的微生物群落结构。在具有龋齿促进饮食的大鼠龋齿模型中,64 mg / L GH12调节了牙菌斑的微生物群,其中龋齿相关细菌的数量减少了,而共生细菌的数量增加了。此外,64 mg / L GH12在所有位置均显着降低了龈沟和光滑表面龋齿的龋齿分数。总之,GH12抑制了牙菌斑的致龋特性,而不会干扰健康个体的牙菌斑微生物,并且GH12调节了致生菌微生物的生态系统并阻止了龋齿的发展。
更新日期:2020-07-02
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