Determining the functions of cancer driver genes in cancer models to mimic in vivo tumors has been a significant challenge. In a recent study, Han et al. implemented large-scale clustered regularly interspaced short palindromic repeats (CRISPR) screening in 3D lung cancer spheroids, revealing advantages of 3D spheroids over 2D monolayers, wherein novel therapeutic targets were identified, such as carboxypeptidase D (CPD).

中文翻译：

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3D 球体推动肿瘤表征。

确定癌症模型中癌症驱动基因的功能以模拟体内肿瘤一直是一项重大挑战。在最近的一项研究中，Han等人。在 3D 肺癌球体中实施大规模成簇规则间隔短回文重复序列 (CRISPR) 筛选，揭示了 3D 球体优于 2D 单层的优势，其中确定了新的治疗靶点，例如羧肽酶 D (CPD)。