Key message

Our study shows for the first time a complete guabiroba micropropagation system with acclimatized plants. BA was the best plant growth regulator for multiplication and IBA for rooting.

Abstract

Campomanesia xanthocarpa O. Berg (Myrtaceae), popularly known as guabiroba, is a woody species native to Brazil, important for its potential as a medicinal plant and fruit tree. For the first time, this study shows a complete system of micropropagation for this species. For this, nodal segments with two axillary buds each were used as starting material. Plant Preservative Mixture™ (0.1%) added to culture media was efficient in controlling contamination throughout the culture process. Two formulations of culture medium were compared for in vitro establishment. 2-Isopentenyladenine, 6-benzyladenine, kinetin and zeatin were tested during the multiplication step. Woody plant medium (WPM) was appropriate for all culture steps and, during the multiplication process, 2.2 µM BA induced the best numbers of new shoots per explant during three subcultures (3.0–3.5 per month). For rooting of microcuttings, a rate of 53% was reached in the WPM supplemented with 4.9 µM indol-3-butyric acid. A plastic box containing a mixture of commercial substrate and vermiculite (1:1 v/v) was used for plantlet acclimatization, allowing 52% of survival after two months. In conclusion, a complete micropropagation protocol was developed providing healthy plants. Further studies are needed to improve multiplication and survival rates.

中文翻译：

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来自巴西大西洋森林的药用树桔梗（Cantomanesia xanthocarpa O. Berg）的微繁殖

关键信息

我们的研究首次显示了具有驯化植物的完整瓜比罗巴微繁殖系统。BA是繁殖的最佳植物生长调节剂，生根的是IBA。

抽象

桔梗O. Berg（桃金娘科），俗称瓜比罗巴（Gubiroba），是巴西原生的木本物种，对于其作为药用植物和果树的潜力至关重要。这项研究首次显示了该物种的完整微繁殖系统。为此，将具有两个腋芽的节段用作起始材料。添加到培养基中的Plant Preservative Mixture™（0.1％）可有效控制整个培养过程中的污染。比较了两种培养基配方以用于体外建立。在乘法步骤中测试了2-异戊烯基腺嘌呤，6-苄基腺嘌呤，激动素和玉米素。木本植物培养基（WPM）适用于所有培养步骤，在繁殖过程中，在三个传代培养期间，每个外植体2.2 µM BA诱导出最佳的新芽数量（每月3.0–3.5）。为了使微碎屑生根，在补充了4.9 µM吲哚-3-丁酸的WPM中达到了53％的比率。使用装有商业基质和ver石（1：1 v / v）混合物的塑料盒来适应小苗的生长，两个月后可以存活52％。总之，已开发出可提供健康植物的完整微繁殖方案。需要进一步研究以提高繁殖和存活率。