Key message

This non-invasive clearing technique allows for deep clear imaging of plant tissue and 3D structural analysis of leaf cells.

Abstract

Optical-clearing technology allows the reduction of light scattering and absorption in internal biological tissues. This technology allows microscopy techniques, such as confocal laser scanning microscopy, to obtain structural information deep within the internal tissue and also allows for three-dimensional (3D) tissue analysis. However, the transparent method in plants remains to be further elucidated. In this study, we first report on a non-invasive, rapid and effective clearing technique that increases the transmission of light through plants. Importantly, we found that the leaf terminal veins of four species had different characteristics based on fluorescence imaging observations. Moreover, through the combination of transparent technology and microscopic observation, we clearly observed the three-dimensional structure of plant leaf cells. Our simple and rapid clearing method is useful for fluorescence imaging the intact internal structure. Taken together, the results of this study will provide valuable information for further microscopic experimental investigations in trees.

中文翻译：

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一种快速有效的光学清除技术，用于树木深层组织荧光成像

关键信息

这种非侵入式清除技术可以对植物组织进行深度清晰的成像，并可以对叶细胞进行3D结构分析。

抽象

光学清除技术可以减少内部生物组织中的光散射和吸收。该技术允许使用显微镜技术（例如共聚焦激光扫描显微镜）获得内部组织深处的结构信息，并且还可以进行三维（3D）组织分析。但是，植物中的透明方法还有待进一步阐明。在这项研究中，我们首先报告了一种非侵入性，快速而有效的清除技术，该技术可增加植物的光传输。重要的是，基于荧光成像观察，我们发现四种物种的叶末端静脉具有不同的特征。此外，通过透明技术和显微镜观察的结合，我们清楚地观察到植物叶细胞的三维结构。我们简单快速的清除方法可用于对完整的内部结构进行荧光成像。两者合计，这项研究的结果将为进一步的树木微观实验研究提供有价值的信息。